沙棘WRI1转录因子基因的生物信息学分析

Bioinformatics Analysis of WRI1 Gene in Hippophae rhamnoides

以构建的沙棘(Hippophae rhamnoides Linn.)不同组织转录组数据库为基础,分离得到1个沙棘WRI1转录因子编码基因,命名为Hr WRI1,利用生物信息学方法对其基因结构、理化性质、保守域、信号肽、亚细胞定位、磷酸化位点和高级结构等进行了预测和较为全面的分析。结果表明,该成员含有1 206 bp的完整开放阅读框,编码401个氨基酸组成的不稳定亲水蛋白,无信号肽和跨膜结构域,存在多个磷酸化位点,定位于细胞核中,高级结构以无规则卷曲为主。与不同植物中已报道的同源WRI1转录因子进行多重序列比对后,发现它们在核酸与氨基酸水平均高度同源,并且具有相同的结构域。

In the present study,a Hr WRI1 gene firstly based on the transcriptome sequencing data from H. rhamnoides was obtained. Then,c DNA and deduced amino acid sequence, physical and chemical characterization,conserved domain,signal peptide,subcellular localization,phosphorylation site molecular modeling were predicted and analyzed. It follows that this predicted c DNA has an open reading frame of 1 206 bp in length,encoding protein of 401 amino acids. It was hydrophilic proteins,no transmembrane regions and signal peptide,contain multiple phosphorylation sites. A subcellular localization analysis predicted that they may exist in the nucleus. In addition,the secondary structure is mainly based on random coil. In comparison to the other known WRI1 from various species,the overall sequence alignment suggested that they were highly similar at the protein level,especially conserved domain. Our investigation could definitely provide a significant foundation for further research on function analysis of Hr WRI1.