低砷岩藻多糖的制备技术

The extraction technology of fucoidan with low arsenic

介绍一种低砷含量的岩藻多糖制备技术：以羊栖菜为原料,在传统酸提岩藻多糖工艺的基础上,增加去除浸泡酸溶液及乙醇沉淀纯化岩藻多糖2个步骤,并对这2个步骤进行条件优化。去除浸泡酸溶液的最佳处理条件为使用5倍体积、浓度1%的柠檬酸溶液浸泡藻体,浸泡温度50℃、浸泡4 h后去除浸泡酸溶液,并使用等量的纯水洗涤一遍,然后补充等量的纯水。乙醇沉淀纯化岩藻多糖的最佳条件是：向浓缩液中加入乙醇,使乙醇浓度达到35%,静置30 min,即可离心去除沉淀,上清液浓缩去除乙醇后直接喷雾造粒。使用优化后的工艺进行提取,岩藻多糖中总砷含量由传统酸提工艺的40 mg/kg降低到（0.5~1）mg/kg,且产品得到进一步纯化,L-岩藻糖含量由20%提高到35%以上。

The preparation technology of fucoJdan with low arsenic was about which included two additional steps on the basis of the traditional acid extraction process using Hijiki as raw materials. The two steps were removing acid soaking solution and adding alcohol to purify fucoidan. The best acid treating condition showed that the Hijiki was soaked in 1% citrate solution for 4 h in 50℃. After soaked, the Hijiki was washed one time with pure water, then removing the wash water and adding the same amount of pure water. The step of purifying fucoidan was like which was adding ethanol to the Hijiki extraction to form a new system with impurity and liquid. It was keeping for 30 min with the ethnol concentration of 35% to seprate out more impurities in the system. The impurities were wiped off through centrifuging and the ethnol was removed through concentrating. Ultimately the total arsenic of fucoidin in （0.5-1）mg/kg, and L-fucose was over 35%. The fucoidan extracted with this process meet the demands of application of the terminal food.