超声波辅助响应面法提取芝麻中芝麻素与芝麻素的结晶

Ultrasonic-assisted extraction of sesamin and sesamin crystallization from sesame by response surface analysis methodology

运用超声辅助乙醇溶液提取芝麻素,在单因素试验基础上运用响应面设计对提取条件进行了优化,并对芝麻素的结晶纯化进行了研究。试验结果表明:3个因素对芝麻素提取率影响的主次顺序依次为料液比>时间>温度。提取温度、时间、料液比的较佳条件分别为45℃、40 min、1:15。在此条件下预测芝麻素的提取率为0.6123%:芝麻素的浓度为1200μg/mL时,最有利于进行结晶纯化。

Sesamin was extracted by ultrasound-assisted enthanol solution. On the basis of single fac- tor experiment, the parameters of ultrasound-assisted extration of sesamin were optimized by response surface analysis methodology. The result indicated that the factors affecting yield of sesamin were as follows： the liquid-solid ratio〉the ultrasonic treament time〉ultrasonic treament temperature. The yield of sesamin was about 0.6123% under the optimal extracting condition, which were the liquid-solid ratio 1 ： 15, the ultrasonic treament time 40 rain, ultrasonic treament temperature 45℃. When the concentration of sesamin was 1200 μg/mL, it was the most favorable for crystallization.