近红外免疫层析法快速检测O1群小川型霍乱弧菌方法的建立与应用

Establishment and application of fast detection of Vibrio cholerae O1 Ogawa based on near-infrared immunochromatography

目的：建立一种快速检测O1群小川型霍乱弧菌的近红外免疫层析方法。方法：利用近红外免疫层析技术,采用双抗体夹心法检测O1群小川型霍乱弧菌,制备靶向于O1群小川型霍乱弧菌的免疫层析试纸条。结果：整个检测方法在45 min内即可完成,检测O1群小川型霍乱弧菌的最低检出浓度为1×10~2 cfu/mL。特异性实验表明,该试纸条与O1群小川型霍乱弧菌样品发生特异性反应,与沙门氏菌、金黄色葡萄球菌、志贺氏菌、单增李斯特菌、副溶血弧菌无交叉反应。与常用的胶体金方法相比,近红外免疫层析法对靶标菌的灵敏度提高了100倍。结论：新研制的O1群小川型霍乱弧菌试纸条具有特异性强、灵敏度高的特点,实验简单、方便,满足口岸现场快速检测的需要,提高检验检疫效率。

Objective： To establish a rapid detection of near-infrared immunochromatographic method of Vibrio cholerae O1 Ogawa. Method： Using near-infrared immunochromatography to detect Vibrio cholerae O1 Ogawa, we use double antibody sandwich method for preparing targeting Vibrio cholerae O1 Ogawa immunochromatographic test strip. Results： The results of the test strip and Vibrio cholerae O1 Ogawa sample have specific reaction and no cross-reaction with Salmonella, Staphylococcus aureus, Shigella, Listeria monocytogenes and Vibrio parahaemolyticus. This method can be completed in 45 min. The minimum detectable concentration detection of Vibrio cholerae O1 Ogawa was 1×10^2 cfu/mL. Compared with colloidal gold method, near-infrared immunochromatography sensitivity of target bacteria 100 times. Conclusion： The newly developed Vibrio cholerae O1 Ogawa test strip has strong specificity and high sensitivity. The experiment is simple and easy to meet the needs of port-site rapid test. This will improve the efficiency of inspection and quarantine.