土茯苓产地加工过程中去皮的合理性分析

Rational Analysis on Peeling in Habitat Processing of Smilacis Glabrae Rhizoma

目的:探讨土茯苓产地加工过程中去皮的合理性,为该药材的产地加工提供理论依据。方法:将新鲜土茯苓分成去皮组、不去皮组、皮组3组,用阴干,晒干和35,55,80℃烘干5种方法干燥加工,利用UV测定总黄酮含量(以芦丁为对照品),检测波长500 nm;利用HPLC测定落新妇苷、白藜芦醇、花旗松素含量,检测波长分别为291,306,289 nm;利用等离子体质谱仪测定铜、铅、镉、汞、砷5种有害重金属含量,比较各成分含量的差异性。结果:土茯苓皮组中总黄酮、落新妇苷、白藜芦醇、花旗松素均远高于去皮组和不去皮组,且不去皮组中上述成分大多高于去皮组;土茯苓皮组中铜和砷远高于去皮组和不去皮组,土茯苓皮组和不去皮组中铅含量远高于去皮组,三者中各元素含量均低于2015年版《中国药典》限量。结论:土茯苓的有效成分和重金属均富集在表皮部分,建议作药用时,不需要去皮;作食用时,为了避免重金属蓄积,可选择去皮。

Objective： To research on rationality of peeling in the habitat processing of Smilacis Glabrae Rhizoma. Method： The fresh Smilacis Glabrae Rhizoma was divided into three groups of peeling group,nonpeeling group and peel group,then they were processed by drying at 35,55,80 ℃,in the shade and the sun,the content of total flavonoids was measured by UV with rutin as reference substance,the contents of taxifolin,astilbin and resveratrol ware determined by HPLC,the contents of five kinds of harmful heavy metals（Cu,Pb,Cd,Hg and As） were measured by ICP-MS,in order to compare the differences between them. Result： The contents of total flavonoids,taxifolin,astilbin and resveratrol in peel group were higher than those in peeling group and non-peeling group,the above ingredients in non-peeling group were higher than those in peeling group. The contents of Cu and As in peel group were higher than those in peeling group and non-peeling group,the content of Pb in peel group and non-peeling group were higher than that in peeling group,all elements of the three groups were lower than the limited values in 2015 edition of Chinese Pharmacopoeia. Conclusion： The effective components and the heavy metals of Smilacis Glabrae Rhizoma are concentrated in the epidermis,for medicinal purposes,you do not need to peel; for edible,in order to avoid heavy metal accumulation,you can choose to peel.