不同盐制方法对牛膝中有效成分含量的影响

Effect of Different Salting Methods on Content of Effective Components in Achyranthis Bidentatae Radix

目的:比较盐粒拌炒和盐水炙牛膝2种不同方法对牛膝中β-蜕皮甾酮,25R-牛膝甾酮和25S-牛膝甾酮含量的影响,为牛膝盐制工艺研究提供参考。方法:采用HPLC测定β-蜕皮甾酮,25R-牛膝甾酮和25S-牛膝甾酮的含量,流动相乙腈-水-甲酸(16∶84∶0.1),检测波长250 nm。以牛膝生品为参考,考察2种盐制方法对牛膝中指标成分含量的影响。结果:盐水炙样品中以每100 g牛膝加食盐1 g,在150~180℃下炒制15 min的含量最高,盐粒拌炒样品以每100 g牛膝加食盐2 g,150~180℃炒制4 min的含量最高。盐水炙样品中β-蜕皮甾酮,25R-牛膝甾酮,25S-牛膝甾酮质量分数均以3号样品为最高,分别为0.083%,0.015 7%,0.010%;盐粒拌炒样品中三者的最高质量分数分别为0.100%,0.019 1%,0.012 3%。结论:盐粒拌炒牛膝的样品中3种甾酮类成分的含量均大于生品和盐水炙牛膝的样品,2种不同盐制方法对牛膝中有效成分均有影响,为牛膝盐制工艺的确定提供参考。

Objective： To compare effect of stir-frying with sized salt and baked with salt solution on contents of β-hydroxyecdysone,25R-inokosterone and 25S-inokosterone in Achyranthis Bidentatae Radix.Method： HPLC was adopted to determine contents of β-hydroxyecdysone,25R-inokosterone and 25S-inokosterone with mobile phase of acetonitrile-water-formic acid（16 ∶ 84 ∶ 0. 1） and detection wavelength of 250 nm. Raw products of Achyranthis Bidentatae Radix was used as reference to examine effect of these two salting technologies on contents of index ingredients. Result： Contents of index ingredients in samples baked with salt solution were the highest when 1 g of salt was included in 100 g of Achyranthis Bidentatae Radix,and being fried for 15 mins at 150-180 ℃; those in samples stir-fried with sized salt were the highest when 2 g of salt was included in 100 g of Achyranthis Bidentatae Radix,and being fried for 4 mins at 150-180 ℃. Contents of β-hydroxyecdysone,25 Rinokosterone and 25S-inokosterone in No. 3 samples were the highest among samples baked with salt solution,which were 0. 083%,0. 015 7% and 0. 010%; the highest contents of these three ingredients of samples stir-fried with sized salt were 0. 100%,0. 019 1%,0. 012 3%,respectively. Conclusion： Contents of three kinds of ketosteroid ingredients in samples stir-fried with sized salt are more than those in raw products and samples baked with salt solution,different salting technologies both have effect on effective ingredients in Achyranthis Bidentatae Radix.