不同发酵方式对神曲消化酶活力及化学成分的影响

Effect of Different Fermentation Way on Digestive Enzyme Activity and Chemical Composition of Massa Medicata Fermentata

目的:优化神曲发酵工艺,比较不同发酵方式对神曲消化酶活性及化学成分的影响。方法:以淀粉酶、蛋白酶活力和HPLC特征图谱为评价指标,对未发酵、恒温恒湿发酵及自然室温发酵神曲进行对比研究,并与市售神曲进行比较;以淀粉酶、蛋白酶活力为指标,通过单因素试验考察发酵相对湿度及药汁加入量。结果:神曲最佳发酵方式以恒温恒湿发酵效果较优,其淀粉酶、蛋白酶活力分别为14.768 mg·min-1·g-1和79.467μg·min-1·g-1,化学成分与自然发酵和Z生品差别不大。与参照图谱相比,相似度除未发酵组<0.9外,其余各组均>0.9;最佳发酵相对湿度80%,麸面中药汁加入量0.65 m L·g-1。结论:恒温恒湿发酵神曲产生的消化酶活力较高,较自然室温发酵快速、质量可控,为神曲的质量标准制定提供参考依据。

Objective： To optimize fermentation process of Massa Medicata Fermentata by investigating its different fermentation way. Method： Using the amylase and protease activity combining with HPLC specific chromatogram as the evaluating indicators,unfermentation,constant temperature and humidity fermentation,natural room temperature fermentation of Massa Medicata Fermentata were compared to optimize the best fermentation way,then do comparative study with commercially available Massa Medicata Fermentata（Z raw product）. Taking the activity of amylase and protease as indicators,single factor tests were carried out on the fermentation humidity and additive amount of herbal medicine juice to optimize the best process. Result： The best fermentation way was constant temperature and humidity fermentation,its activities of amylase and protease were14. 768 mg·min-1·g-1and 79. 467 μg·min-1·g-1,its chemical composition was similar to natural fermentation and Z raw product. Compared with reference chromatogram,the similarity values were all higher than 0. 9 except the unfermentation group. The best fermentation humidity was 80% and the best additive amount of herbal medicine juice was 65 m L for 100 g of bran-flour. Conclusion： The digestive enzyme activity of Massa Medicata Fermentata fermented with constant temperature and humidity is high,its fermentation speed is faster than the natural room temperature fermentation,its fermentation process is controllable and feasible,which can provide a scientific reference for formulating quality standard of Massa Medicata Fermentata in future.