真菌诱导子对怀地黄组培苗生长及次生代谢产物合成的影响

Effect of Fungal Elicitors on Growth and Secondary Metabolites Content in Rehmannia glutinosa Tissue Culture

目的:研究分离自地黄不同部位的内生真菌诱导子对怀地黄组培苗生长及其次生代谢产物含量的影响,初步探究其促生作用。方法:以2株Alternaria属内生真菌A.alternata,Penicillium属内生真菌P.axalicum制备不同类型的诱导子,发酵液滤液和菌丝降解液,分别考察其对地黄组培苗株高、鲜重、叶绿素含量、梓醇及毛蕊花糖苷含量的影响,并用SPSS 17.0进行数据分析。高效液相色谱法测定梓醇及毛蕊花糖苷含量。结果:不同菌株制备的真菌诱导子促生作用差异明显,以Penicillium属内生真菌P.axalicum制备的真菌诱导子对怀地黄组培苗促生效果较佳,且以其发酵液滤液促生效果最好。在该培养条件下,怀地黄组培苗株高、鲜重、根长、总叶绿素、梓醇及毛蕊花糖苷含量增加率分别为27.80%,29.49%,22.08%,15.05%,35.98%和68.18%。结论:内生真菌诱导子可促进怀地黄组培苗生长,并提高其次生代谢产物含量,在今后地黄内生真菌应用中可做进一步探究。

Objective： To study the effects of endophytic fungal elicitors,which were separated from different parts of Rehmannia glutinosa,on growth and secondary metabolites contents,in R. glutinosa tissue culture,and make a preliminary study on growth-promoting role of endophytic fungal elicitor. Method： Alternaria alternata,and Penicillium axalicum were used to prepare two different types of elicitors,filtered fermentation liquor and mycelium degradation solution. Then their effects on plant height,fresh weight,chlorophyll content,catalpol and acteoside contents in R. glutinosa tissue culture were investigated. The data analysis was done by using SPSS.The contents of catalpol and acteoside were detected by HPLC. Result： Different fungal elicitors showed significant differences in growth-promoting role. The fungal elicitors of P. axalicum showed a better promoting role than others,and its fermentation filtrate showed the best effect. In this condition,the plant height,fresh weight,root length,chlorophyll content,catalpol and acteoside contents of R. glutinosa tissue culture increased respectively by27. 80%,29. 49%,22. 80%,15. 05%,35. 98% and 68. 18%. Conclusion： Endophytic fungal elicitor of R. glutinosa can promote the growth of tissue culture,and improve the contents of its secondary metabolites.Further studies are needed for endophytic fungal applications in R. glutinosa.