中药半枝莲中二萜类生物碱scutebarbatine A的分离及含量测定

Isolation and Determination of Scutebarbatine A in Scutellariae Barbatae Herba

目的:半枝莲中生物碱scutebarbatine A的分离鉴定及其含量测定方法的建立。方法:采用色谱分离方法从半枝莲中分离得到scutebarbatine A,并通过光谱技术确定其结构;色谱条件为Agilent-Eclipse XDB-C18色谱柱(4.6 mm×250 mm,5 mm),流动相甲醇-水(60∶40),流速1 m L·min-1,检测波长260 nm,柱温40℃,考察了不同产地半枝莲中scutebarbatine A的含量差异。结果:Scutebarbatine A线性范围为0.030 34~1.213 6μg(r=0.999 9),重复性RSD 1.8%,精密度RSD 0.5%,供试品溶液在16 h内稳定,平均加样回收率为100.2%,RSD 3.6%。结论:从半枝莲中分离鉴定scutebarbatine A,所建立的定量方法可控、科学,可用于该药材的质量控制。

Objective： To isolate and identify the contents of scutebarbatine A from Scutellariae Barbatae Herba. Method： Scutebarbatine A was isolated from Scutellariae Barbatae Herba by chromatographic separation method and its structure was determined by spectroscopic method. Agilent-Eclipse XDB-C18column（4. 6 mm × 250 mm,5 mm） was used with methanol-water（60 ∶ 40） as the mobile phase at a flow rate of 1 m L·min-1. The detection wavelength was set at 260 nm and the column temperature was 40 ℃. This method was used to investigate the differences of scute barbatine A contents in Scutellariae Barbatae Herba of different origins.Result： The calibration curves of scutebarbatine A showed good linearity over the ranges of 0. 030 34-1. 213 6 μg（r = 0. 999 9）. The repeatability RSD was 1. 8%; precision RSD was 0. 5%; and the test sample solution was stable within 16 h,with an average recovery rate of 100. 2%（RSD 3. 6%）. Conclusion： The quantitative determination method to isolate and identify scutebarbatine A from scutellariae Barbatae Herba is controllable,scientific,and suitable for the quality control of Scutellariae Barbatae Herba.