摘要
目的:研究茵陈配方颗粒抗炎活性部位的化学成分。方法:采用重结晶及硅胶,羟丙基葡聚糖凝胶(Sephadex LH-20),半制备HPLC等色谱方法进行分离纯化,依据波谱学方法及理化性质鉴定化合物结构。结果:对茵陈配方颗粒抗炎活性部位(大孔树脂50%乙醇部位和无水乙醇部位)进行系统化学成分研究,分离并鉴定了13个化合物,分别为对羟基苯乙酮(1),2,5-二羟基桂皮酸乙酯(2),槲皮素(3),木犀草素(4),7S,8R-二氢去氢双松柏醇(5),洋李素(6),7,4'-二甲氧基山柰酚(7),槲皮素-7-O-α-L-鼠李糖苷(8),9S,12S,13S-三羟基-反-10-十八烯酸甲酯(9),异鼠李素-4'-O-β-D-葡萄糖苷(10),山柰酚-3-O-β-D-葡萄糖苷(11),硬脂酸(12)和β-胡萝卜苷(13)。结论:以上13个化合物均为首次从茵陈配方颗粒中分离得到,其中,化合物9~11为首次从蒿属中分离得到,化合物2和5为首次从滨蒿中分离得到。
Objective: To study the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba. Method: The compounds were isolated and purified by recrystallization,silica gel,Sephadex LH-20,and semi-preparative HPLC. Their structures were identified on the basis of spectroscopic methods combined with physicochemical characteristics. Result: From the systemic analysis on the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba(macroporous resin 50% ethanol,and 100% ethanol),thirteen compounds were isolated from the antiinflammatory fractions and were identified as 4'-hydroxyacetophenone(1),2,5-dihydroxycinnamic acid ethyl ester(2),quercetin(3),luteolin(4),7S,8R-dihydrodehydroconiferyl alcohol(5),prudomestin(6),7,4'-dimethoxykaempferol(7),quercetin-7-O-α-L-rhamnoside(8),9S,12 S,13S-trihydroxy-10E-octadecenoate(9),isorhmnetin-4'-O-β-D-glucoside(10),kaempferol-3-O-β-D-glucoside(11),stearic acid(12) and β-daucosterol(13) respectively. Conclusion: All the above 13 compounds were obtained from the formula granules of Artemisiae Scopariae Herba for the first time; compounds 9-11 were obtained from genus Artemisia for the first time; and compounds 2 and 5 were isolated from Artemisia scoparia for the first time.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第3期55-59,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医临床研究基地业务建设科研专项(JDZX2012057)