茵陈配方颗粒中的化学成分分离鉴定

Chemical Constituents from Formula Granules of Artemisiae Scopariae Herba

目的:研究茵陈配方颗粒抗炎活性部位的化学成分。方法:采用重结晶及硅胶,羟丙基葡聚糖凝胶(Sephadex LH-20),半制备HPLC等色谱方法进行分离纯化,依据波谱学方法及理化性质鉴定化合物结构。结果:对茵陈配方颗粒抗炎活性部位(大孔树脂50%乙醇部位和无水乙醇部位)进行系统化学成分研究,分离并鉴定了13个化合物,分别为对羟基苯乙酮(1),2,5-二羟基桂皮酸乙酯(2),槲皮素(3),木犀草素(4),7S,8R-二氢去氢双松柏醇(5),洋李素(6),7,4'-二甲氧基山柰酚(7),槲皮素-7-O-α-L-鼠李糖苷(8),9S,12S,13S-三羟基-反-10-十八烯酸甲酯(9),异鼠李素-4'-O-β-D-葡萄糖苷(10),山柰酚-3-O-β-D-葡萄糖苷(11),硬脂酸(12)和β-胡萝卜苷(13)。结论:以上13个化合物均为首次从茵陈配方颗粒中分离得到,其中,化合物9~11为首次从蒿属中分离得到,化合物2和5为首次从滨蒿中分离得到。

Objective： To study the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba. Method： The compounds were isolated and purified by recrystallization,silica gel,Sephadex LH-20,and semi-preparative HPLC. Their structures were identified on the basis of spectroscopic methods combined with physicochemical characteristics. Result： From the systemic analysis on the chemical constituents of the anti-inflammatory fractions from the formula granules of Artemisiae Scopariae Herba（macroporous resin 50% ethanol,and 100% ethanol）,thirteen compounds were isolated from the antiinflammatory fractions and were identified as 4＇-hydroxyacetophenone（1）,2,5-dihydroxycinnamic acid ethyl ester（2）,quercetin（3）,luteolin（4）,7S,8R-dihydrodehydroconiferyl alcohol（5）,prudomestin（6）,7,4＇-dimethoxykaempferol（7）,quercetin-7-O-α-L-rhamnoside（8）,9S,12 S,13S-trihydroxy-10E-octadecenoate（9）,isorhmnetin-4＇-O-β-D-glucoside（10）,kaempferol-3-O-β-D-glucoside（11）,stearic acid（12） and β-daucosterol（13） respectively. Conclusion： All the above 13 compounds were obtained from the formula granules of Artemisiae Scopariae Herba for the first time; compounds 9-11 were obtained from genus Artemisia for the first time; and compounds 2 and 5 were isolated from Artemisia scoparia for the first time.