HPLC同时测定维吾尔族药复方小艾飞中5种有效成分的含量

Content Determination of 5 Kinds of Effective Components in Uighur Medicine Compound Xiaoaifei by HPLC

目的:建立HPLC同时测定复方小艾飞中槲皮素、山柰素、乔松素、高良姜素4种黄酮类成分及胡椒碱含量的方法。方法:采用Hypersil BDS C18色谱柱(4.6 mm×250 mm,5μm),以乙睛(A)-1%磷酸溶液(B)-甲醇(C)为流动相,梯度洗脱(0~12 min,28%A∶52%B∶20%C;12~13 min,28%~30%A,52%~50%B,20%C;13~45 min,30%A∶50%B∶20%C),流速1.0 m L·min-1,柱温30℃,进样量20μL,检测波长280 nm。结果:槲皮素、山柰素、乔松素、高良姜素、胡椒碱的标准曲线分别为Y=357 314X-88 066(r=0.999 9),Y=442 853X-74 204(r=0.999 9),Y=647 926X-327 436(r=0.999 9),Y=471 672 X-256 408(r=0.999 9),Y=290 000X-391 906(r=0.999 9),线性关系良好。平均加样回收率(n=9)分别为98.4%(RSD 1.6%),98.9%(RSD 1.7%),101.3%(RSD 2.2%),98.6%(RSD 2.1%),98.6%(RSD 1.8%)。结论:该方法准确、简便,具有良好的重复性,可用于维吾尔族药复方小艾飞的质量控制。

Objective： To establish a method for simultaneous contents determination of 4 flavonoids including quercetin,kaempferide,pinocembrin,and galangin as well as alkaloids piperine in Uighur medicine compound Xiaoaifei by using HPLC. Method： Separation was performed on Hypersil BDS C18（4. 6 mm ×250 mm,5 μm） column with acetonitrile（A）-1% phosphoric acid（B）-methanol（C）（0-12 min,28% A ∶52% B ∶ 20% C; 12-13 min,28%-30% A,52%-50% B,20% C; 13-45 min,30% A ∶ 50% B ∶ 20% C） as the mobile phase for gradient elution at a flow rate of 1. 0 m L·min-1. The detection wavelength was set at 280 nm; the column temperature was 30 ℃,and the sample size was 20 μL. Result： Y = 357 314X-88 066（r = 0. 999 9）,Y = 442 853X-74 204（r = 0. 999 9）,Y = 647 926X-327 436（r = 0. 999 9）,Y = 471 672X-256 408（r =0. 999 9）,and Y = 290 000X-391 906（r = 0. 999 9）,respectively in the calibration curves of quercetin,kaempferide,pinocembrin,galangin and piperine,showing good linear relationship. The mean recoveries（n = 9）of them were 98. 4%（RSD 1. 6%）,98. 9%（RSD 1. 7%）,101. 3%（RSD 2. 2%）,98. 6%（RSD 2. 1%） and98. 6%（RSD 1. 8%）,respectively. Conclusion： The developed method is simple,accurate,reliable,and helpful to control the quality of Uighur medicine compound Xiaoaifei.