柿树寄生总提取物对链脲佐菌素致小鼠糖尿病的保护作用及机制的影响

Protective Effect and Mechanism of Total Extract From Persimmon Parasitism on Diabetic Mice Induced by Streptozotocin

目的:观察柿树寄生总提取物对链脲佐菌素(STZ)所致小鼠糖尿病的影响。方法:制备柿树寄生总提取物;小鼠尾静脉注射STZ(120 mg·kg-1)建立糖尿病小鼠模型。糖尿病小鼠随机分为5组:柿树寄生总提取物高、中、低剂量(10,5,2.5 g·kg-1)组,模型组,二甲双胍组;另设10只正常小鼠作为空白组。各组小鼠均以连续给药14 d后,分别测定各小鼠空腹血糖(FBG),肝组织和血清中丙二醛(MDA)水平及超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活力值,以及血脂代谢情况;并通过HE染色观察胰腺组织的病理学变化。结果:与模型组比较,柿树寄生总提取物对STZ所致糖尿病小鼠有明显降低血糖的作用,明显提高肝组织和血清的SOD,GSH-Px活力,降低MDA含量(P<0.05);降低血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白(LDLC)水平和提高高密度脂蛋白(HDLC)含量(P<0.05);改善胰腺组织病理学变化。结论:柿树寄生总提取物能明显降低STZ致小鼠糖尿病模型的血糖水平,增强机体的抗氧化能力以及改善血脂代谢异常情况。

Objective： To observe the effects of total extract from persimmon parasitism（EPP） in diabetic mice induced by streptozotocin（STZ）. Method： EPP was extracted and diabetic mice models were established by tail vein injection of STZ（120 mg·kg）. Diabetic mice were randomly divided into 5 groups： EPP high dose group,EPP middle dose group,EPP low dose group,model control group,and metformin（10,5,2. 5 g·kg-1） group; another 10 healthy mice were considered as normal control group. After the mice were treated with corresponding drugs for 14 days,their fasting blood glucose（FBG）,the activity of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px） and malonaldehyde（MDA） levels in serum and liver tissues,as well as lipid metabolism were determined（P 0. 05）. The pathological changes of pancreas tissues were observed through HE staining. Result： As compared with the model control group,EPP could significantly decrease the blood glucose in STZ-induced diabetic mice and decrease MDA content,but significantly increase the levels of SOD and GSH-Px in serum and liver tissues; reduce the levels of cholesterol（TC,triglyceride（TG）,and low density lipoprotein（LDLC） in serum; increase the level of high density lipoprotein（HDLC）. In addition,there was an improvement on pathological changes of pancreas in EPP groups. Conclusion： EPP can significantly reduce fasting blood glucose level in diabetic mice induced by STZ,improve the ability of anti-oxidation and lipid metabolism.