摘要
目的:观察柿树寄生总提取物对链脲佐菌素(STZ)所致小鼠糖尿病的影响。方法:制备柿树寄生总提取物;小鼠尾静脉注射STZ(120 mg·kg-1)建立糖尿病小鼠模型。糖尿病小鼠随机分为5组:柿树寄生总提取物高、中、低剂量(10,5,2.5 g·kg-1)组,模型组,二甲双胍组;另设10只正常小鼠作为空白组。各组小鼠均以连续给药14 d后,分别测定各小鼠空腹血糖(FBG),肝组织和血清中丙二醛(MDA)水平及超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活力值,以及血脂代谢情况;并通过HE染色观察胰腺组织的病理学变化。结果:与模型组比较,柿树寄生总提取物对STZ所致糖尿病小鼠有明显降低血糖的作用,明显提高肝组织和血清的SOD,GSH-Px活力,降低MDA含量(P<0.05);降低血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白(LDLC)水平和提高高密度脂蛋白(HDLC)含量(P<0.05);改善胰腺组织病理学变化。结论:柿树寄生总提取物能明显降低STZ致小鼠糖尿病模型的血糖水平,增强机体的抗氧化能力以及改善血脂代谢异常情况。
Objective: To observe the effects of total extract from persimmon parasitism(EPP) in diabetic mice induced by streptozotocin(STZ). Method: EPP was extracted and diabetic mice models were established by tail vein injection of STZ(120 mg·kg). Diabetic mice were randomly divided into 5 groups: EPP high dose group,EPP middle dose group,EPP low dose group,model control group,and metformin(10,5,2. 5 g·kg-1) group; another 10 healthy mice were considered as normal control group. After the mice were treated with corresponding drugs for 14 days,their fasting blood glucose(FBG),the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and malonaldehyde(MDA) levels in serum and liver tissues,as well as lipid metabolism were determined(P 0. 05). The pathological changes of pancreas tissues were observed through HE staining. Result: As compared with the model control group,EPP could significantly decrease the blood glucose in STZ-induced diabetic mice and decrease MDA content,but significantly increase the levels of SOD and GSH-Px in serum and liver tissues; reduce the levels of cholesterol(TC,triglyceride(TG),and low density lipoprotein(LDLC) in serum; increase the level of high density lipoprotein(HDLC). In addition,there was an improvement on pathological changes of pancreas in EPP groups. Conclusion: EPP can significantly reduce fasting blood glucose level in diabetic mice induced by STZ,improve the ability of anti-oxidation and lipid metabolism.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第3期134-138,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81160533
81360129)
南宁市青秀区科学研究与技术开发计划项目(2013S12)
2015年广西研究生教育创新计划项目(YCBZ2015035)
关键词
柿树寄生总提取物
糖尿病
血糖
血脂
抗氧化
total extract from persimmon parasitism
diabetes mellitus
blood glucose
lipid
anti-oxidant