隔山香正丁醇部位对大鼠胸主动脉的舒张作用及机制的影响

Relaxation Effects and Mechanisms of n-Butanolic Fraction of Lemonfragrant Angelica Root on Rat Thoracic Aorta

目的:研究隔山香(Lemonfragrant Angelica Root,LAR)正丁醇部位对大鼠胸主动脉环的舒张作用,并探讨其可能的作用机制。方法:采用离体大鼠胸主动脉血管环灌流方法,考察0.34,0.65,1.22 g·L-1隔山香正丁醇部位对氯化钾(KCl)和去甲肾上腺素(norepinephrine,NE)预收缩的血管环张力的影响。在无Ca2+Kreb’s液中,考察0.34,0.65,1.22 g·L-1隔山香正丁醇部位对氯化钙(Ca Cl2)量效曲线的影响和NE预收缩的血管环张力的影响。激光共聚焦扫描显微镜检测平滑肌细胞内[Ca2+]i的变化。结果:隔山香正丁醇部位对KCl和NE预收缩的内皮完整和去除内皮血管环均具有浓度依赖性的舒张作用(P<0.01),并且去除内皮后的舒张作用未受影响;无Ca2+条件下,隔山香正丁醇部位能使Ca Cl2,NE预收缩强度显著性降低(P<0.01)。激光共聚焦扫描显微镜下,隔山香正丁醇部位(1.22 g·L-1)能抑制KCl,NE和毒胡萝卜素(thapsigargin,TG)引起的平滑肌细胞内[Ca2+]i升高。结论:隔山香正丁醇部位对大鼠胸主动脉血管有一定的舒张作用,可能通过抑制外钙内流和内钙释放发挥作用。本实验为隔山香水蒸气蒸馏后煎煮入药的成药制备工艺和水溶性药效指标成分的分离提供了依据。

Objective： To investigate the relaxation effects and mechanisms of n-butanolic fraction of Lemonfragrant Angelica Root（LAR） on rat thoracic aorta. Method： Thoracic aortic ring perfusion in isolated ratswas used to observe the effect of n-butanolic fraction on tension changes of aorta rings preshrunk by KCl and norepinephrine（NE）. In Ca2 ＋-free kreb ＇s solution,the effects of n-butanolic fraction on Calcium chloride concentration-response curves and the tension changes shrunk by NE were investigated. Confocal laser scanning microscope was used to detect the changes of smooth muscle cells（VSMCs） [Ca2 ＋]i. Result： The n-butanolic fraction of LAR relaxed the KCl and NE-induced contraction of aortic rings,either with or without intact endothelium,in a concentration-dependent manner,and there was no effect on relaxation after after endotheliumdenuding. In Ca2 ＋-free solution,n-butanolic fraction of LAR significantly reduced the Ca Cl2 and NE-induced contraction of aortic rings. Under confocal laser scanning microscope,n-butanolic fraction of LAR（1. 22 g·L-1）could inhibit the increase of smooth muscle cells（VSMCs） [Ca2 ＋]iinduced by KCl,NE and thapsigargin（TG）.Conclusion： n-Butanolic fraction of LAR may relax the tension of aorta rings,probably by inhibiting the influx calcium and calcium release. This study provided evidence for the production techniques of LAR decoction after steam distillation and separating the water-soluble efficacy mark components.