波棱瓜子有效组分配伍对刀豆蛋白A所致小鼠免疫性肝损伤的保护作用

Protective Effect of Active Components Compatibility of Herpetospermum caudigerum on Immunological Liver Injury Induced by Concanavalin A in Mice

目的:探究波棱瓜子中活性成分乙酸乙酯提取物(HE)与脂肪油(HSO)配伍对刀豆蛋白A(Con A)诱导的免疫性肝损伤小鼠的保护作用及其机制。方法:将小鼠80只随机区组分为8组,即空白组,模型组,联苯双酯组(阳性组),HSO组,HE组,组分配伍1∶1,1∶2,2∶1组,每组10只。治疗组分别灌胃(ig)给予波棱瓜子HE,HSO及二者不同剂量配伍药液连续8 d,采用尾静脉一次性注射Con A 30 mg·kg-1诱导造成小鼠免疫性肝损伤模型。造模8 h后采血,制备肝匀浆。计算小鼠的肝脾系数;酶联免疫法(ELISA)检测小鼠血清谷丙转氨酶(ALT),谷草转氨酶(AST),一氧化氮(NO)及肝脏匀浆中超氧化物歧化酶(SOD),丙二醛(MDA)等生化指标的活性及含量;同时对肝组织进行病理学检查。结果:波棱瓜子各治疗组均可降低免疫性肝损伤小鼠的肝脾脏器指数;降低小鼠肝损伤血清ALT,AST水平及NO的含量;增强肝匀浆中SOD活性,降低MDA的含量;减轻Con A对肝组织的病理损伤。其中有效组分HSO-HE 2∶1配伍组各指标较模型组均有显著性差异,护肝作用最佳。结论:波棱瓜子HE与HSO配伍护肝作用优于单一组分,提示配伍时具有协同增效的作用,其保肝机制可能与其抗炎、抗氧化以及清除自由基有关。

Objective： To explore the protective effect and mechanism of active components compatibility of ethyl acetate extracts（HE） and fatty oil（HSO） of Herpetospermum caudigerum on immunologic liver injury induced by concanavalin A（Con A） in mice. Method： The 80 mice were randomly divided into 8groups（n = 10）,normal group,model group,biphenyl double ester group（positive control）,HSO group,HE group,HSO and HE of 1∶ 1,1∶ 2,2∶ 1 ratio groups. After ig administration of ethyl acetate extracts,fatty oil and different compatibilities for 8 days in treatment group,the mice were injected with Con A（30 mg·kg-1） through tail vein to establish the model of immunological liver injury. Blood was collected after 8 hours modeling to prepare liver homogenate. Liver and spleen indexes were calculated; the contents of alanine aminotransferase（ALT）,aspartate aminotransferase（AST） and nitrogen monoxidum（NO） in serum,superoxide dismutase（SOD）,malondialdehyde（MDA） in liver homogenate were assayed by ELISA; and pathological morphological changes in liver cells were monitored by HE. Result： Ethyl acetate extracts,fatty oil and their combinations of H.caudigerum could decrease liver and indexes,the levels of ALT,AST,NO,MDA and increased SOD activities.Meanwhile,the pathological damage in liver was relieved. As compared with the model group,thefatty oiland ethyl acetate extracts at 2 ∶ 1 ratio group showed the best effect on immunological liver injury in mice,with significant differences in above indexes. Conclusion： Ethyl acetate extracts compatibility with oil can protect immunological liver injury in mice,and the effect was superior to that of single component. The mechanism may be associated with anti-inflammatory,anti-oxidation and scavenging oxygen free radical.