氧化应激损伤与HEI-OC1细胞株中Prestin表达的相关性分析

Prestin Expression in HEI-OC1 Cells With Oxidative Stress Damage

目的观察氧化应激损伤对HEI-OC1细胞Prestin表达的影响,探讨感音性聋的发生机制。方法采用不同浓度(50、100、200μM)双氧水(H_2O_2)染毒培养的HEI-OC1细胞(染毒组),对照组细胞加入无药物培养液,24h后检测各组细胞超氧化物歧化酶(SOD)活力,实时荧光定量PCR检测Prestin mRNA表达水平,免疫荧光法分析Prestin表达水平。结果与对照组相比,氧化应激损伤后染毒组的HEI-OC1细胞SOD活力明显下降,尤以200μM H_2O_2染毒组下降更明显(F=9 926.293,P<0.01);氧化应激损伤的HEI-OC1细胞Prestin mRNA和Prestin蛋白表达水平均明显下降,200μM H_2O_2染毒组下降更明显(F=4 065.046、7 657.217,P<0.01)。结论氧化应激损伤可抑制Prestin的表达,此为感音性聋的重要病理学变化。

Objective To examine the effects of oxidative stress induced damage to the Prestin expression in HEI-OC1 cells,and to study the mechanism of sensory deafness.Methods We used different concentrations（50μM,100μM,200μM）of hydrogen peroxide canister to cultivate HEI-OC1 cells,and to detect the activity of superoxide dismutase（SOD）.The quantitative real-time PCR and immunofluorescence were used to detect the prestin expression of mRNA.Results The SOD activity decreased in the HEI-OC1 cells damaged by oxidative stress.The high concentration of the infected group decreased more significantly（F=9926.293,P〈0.01）.The expressions of Prestin mRNA and Prestin protein were decreased obviously in the HEI-OC1 cells.The high concentration of infected group decreased more significantly（F=4065.046 and7657.217,P〈0.01）.Conclusion Oxidative stress inducing damage inhibits the expression of prestin.Prestin protein may be used as a molecular marker of sensory deafness.