基于离子液体辅助氯霉素分子印迹聚合物的制备及应用

Preparation and Application of Molecularly Imprinted Polymers for Chloramphenicol Based on Ionic Liquids Assistance

以氯霉素(CAP)为模板,2-乙烯基吡啶(2-Vp)为功能单体,四氢呋喃和离子液体1-丁基-3-甲基咪唑四氟硼酸盐[BMIm]BF4的混合溶液为反应溶剂,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,偶氮二异丁腈(AIBN)为引发剂,合成了氯霉素的分子印迹及非印迹聚合物。优化功能单体、不同溶剂对印迹聚合物吸附性能的影响,结果表明,以2-乙烯基吡啶为功能单体,四氢呋喃和离子液体[BMIm]BF4(体积比1∶1)作为反应溶剂合成的分子印迹聚合物对氯霉素具有高的吸附容量,良好的特异性识别性能。氯霉素分子印迹聚合物的印迹因子为2.6,进行吸附-解吸附循环5次后,氯霉素印迹聚合物的性能稳定,可重复使用。将制备的氯霉素分子印迹聚合物作为富集材料,应用于鸡蛋样品中氯霉素的检测,回收率可达62.3%~81.1%,准确性好。

In this work,the molecularly imprinted polymers（MIPs） for chloramphenicol（CAP）（CAP-MIPs） were prepared by ionic liquids assistance using 2-vinylpyridine as functional monomer,ethylene glycol dimethacrylate（EGDMA） as the cross-linker and 2,2′-azobisisobutyronitrile（AIBN） as the initiator in the mixture of tetrahydrofuran and ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate（[BMIm]BF4） as the porogenic solvent.The effects of functional monomer,progenic solvent on the binding capacity of MIPs were optimized.The MIPs possessed a high capacity and a favorable selectivity for CAP in the preparation of MIPs using 2-vinylpyridine as functional monomer and ionic liquid（[BMIm]BF4）（1∶1,by volume） as polymeric solvent.The imprinting factor of CAP-MIPs reached to 2.6.The reusability of CAP-MIPs without any deterioration in capacity was demonstrated for five adsorption-desorption cycles.The developed method was applied in the enrichment and determination of CAP in spiked egg samples using CAP-MIPs as the adsorbent,with CAP recoveries of 62.3%-81.1%.