猪肉中β-受体激动剂AlphaLISA检测方法的建立

Quantitative Determination of β-Agonists in Pork Using the Amplified Luminescent Proximity Homogeneous Assay

建立了快速检测猪肉中克伦特罗、沙丁胺醇、溴布特罗和特布他林4种β-受体激动剂的光激化学发光纳米均相时间分辨荧光免疫(Alpha LISA)分析方法。样品经乙酸铵和β-葡萄糖醛酸酶/硫酸酯酶酶解提取过柱,浓缩至干后,用缓冲溶液定容。样品与生物素化抗原、抗体、供体微珠和受体微珠进行酶联免疫,Alpha LISA进行检测,基质标准曲线定量。结果表明:沙丁胺醇、克伦特罗、溴布特罗和特布他林在0.1~500ng/m L范围内呈良好线性,相关系数均大于0.98,沙丁胺醇与克伦特罗、溴布特罗和特布他林的交叉反应率分别为143.3%,179.2%和95.6%,检出限为0.03~0.08 ng/m L。在0.5,10,20μg/kg 3个添加水平下,4种化合物的平均回收率为82.5%~115.9%,相对标准偏差(RSD)均小于12.0%。该方法操作简单,快速准确,适用于猪肉中β-受体激动剂的筛选与检测。

An amplified luminescent proximity homogeneous assay（AlphaLISA） was developed for the determination of four kinds of β agonists（clenbuterol,salbutamol,brombuterol,terbutaline） in pork.The analytes were stepwise extracted with ammonium acetate and β-glucuronidase/sulfatase,the extract was columned and concentrated to dryness,redisolved in buffer.Samples were reacted with botinylated antigen,antibody,donor beads and acceptor beads,and detected by AlphaLISA with matrix standard curve.As a result,there are good linear relationships in the concentration range of 0.1-500 ng/mL,with correlation coefficients larger than 0.98.The cross-reactivities for clenbuterol,brombuterol,terbutaline were 143.3%,179.2%and 95.6%,respectively,and the limits of detection（LOD） were 0.03-0.08 ng/mL.The average recoveries of four compounds at spiked levels of 0.5,10,20 μg/kg were in the range of 82.5%-115.9%with relative standard deviations（RSD） not less than 12.0%.The method is simple,rapid and accurate,and is suitable for the detection and screening of β-agonists in pork.