液液分散微萃取/GC-MS法测定婴幼儿一次性用品中5种致敏原物质

Determination of 5 Allergens in Disposable Product for Infants by Liquid-Liquid Micro-extraction Combined with GC-MS

建立了同时测定婴幼儿一次性用品中5种致敏原物质(柠檬烯、沉香醇、柠檬醛、乙酸苄酯和肉桂醛)的分散液液微萃取/气相色谱-质谱(DLLME/GC-MS)检测方法。样品经乙腈浸泡提取、水分散和盐析后用四氯化碳萃取浓缩,采用GC-MS测定,外标法定量。目标物在0.01~10 mg/kg范围内线性关系良好,相关系数在0.99以上。方法检出限为0.01~0.1 mg/kg。加标回收率为78.7%~106%,相对标准偏差为2.4%~11.3%。该方法前处理简单,灵敏度高,可满足纸尿裤、婴儿专用湿巾等一次性婴幼儿用品中5种致敏原物质的检测要求。

A new method was developed for the determination of 5 allergens （ cinene, linalool, cit- ral, benzyl acetate, cinnamaldehyde） in disposable product for infants by liquid -liquid micro-ex- traction combined with GC - MS. Samples were extracted with acetonitrile. The extract was dispersed with water, salted out with NaC1, and concentrated with tetrachloromethane （CC]4）. The analytes were detected by GC -MS with the external standard method. The calibration curves were linear in the range of 0.01 - 10 mg/kg, wtih correlation coefficients more than 0. 99. The detection limits were in the range of 0. 01 -0. 1 mg/kg. The spiked recoveries were in the range of 78.7% -106% , with RSDs of 2.4% - 11.3%. The result comfirmed that the method was accurate, rapid and sensi- tive, and was suitable for the determination of 5 allergens in disposable product for infants.