不同雌激素水平大鼠牙周炎模型牙龈中IL-1β和COX-2基因的表达及意义

Expression and significance of IL-1β and COX-2 in the gingiva tissues in rat periodontitis model with different estrogen levels

目的:检测白介素(IL)-1β基因和COX-2基因在不同雌激素水平大鼠牙周炎模型牙龈组织中的表达情况,分析雌激素水平对牙周炎的影响。方法:选取40只雌性Wister大鼠随机分为4组,正常对照组(n=10)、牙周炎组(n=10)、去势牙周炎组(n=10)和雌激素治疗组(n=10),采用RT-PCR方法检测各组大鼠牙龈中IL-1β和COX-2的基因表达。结果:IL-1β和COX-2表达强度在雌激素治疗组大鼠牙龈中显著低于去势牙周炎组(P<0.05)。结论:雌激素可显著下调IL-1β、COX-2基因表达,进而减轻牙周炎症状。

Objective： To explore the expressions of IL-1β and COX-2 in the gingiva tissues in rat periodontitis model with different estrogen levels and the effect of estrogen level on periodontitis. Methods： A total of 40 female Wistar rats were randomized into 4 groups,i. e. normal control group（ n = 10）,periodontitis group（ n = 10）,castraction periodontitis group（ n = 10）,and estrogen therapy group（ n = 10）. RT-PCR was used to detect the expressions of IL-1β and COX-2 in the gingiva tissues in each group. Results： The expression intensity of IL-1β and COX-2 in the estrogen therapy group was significantly lower than that in the castraction periodontitis group（ P〈0. 05）. Conclusions： Estrogen can significantly down regulate the expressions of IL-1β and COX-2 in order to alleviate the symptoms of periodontitis.