2型糖尿病不同尿白蛋白排泄率患者血清中miR-342-5p、miR-423-5p的表达量及临床意义

Serum expression and clinical significance of miR-342-5p and miR-423-5p in type 2 diabetes mellitus patients with different urinary albumin excretion rate

目的:研究2型糖尿病不同尿白蛋白排泄率患者血清中miR-342-5p、miR-423-5p的表达量及临床意义。方法:选择2013年5月~2015年12月在我院接受治疗的126例2型糖尿病患者,并根据尿白蛋白/肌酐比值(ACR)分为正常尿蛋白组(NA组)、微量尿蛋白组(MI组)、大量尿蛋白组(MA)组;另取50例健康志愿者作为对照组。采集血清并检测miR-342-5p、miR-423-5p的表达量以及p38MAPK、TGF-β1信号通路分子的含量。结果:NA组、MI组、MA组血清中miR-342-5p和miR-423-5p的表达量均显著高于对照组且ACR越高、血清中miR-342-5p和miR-423-5p的表达量越高;miR-342-5p和miR-423-5p高表达患者血清中MAPKKK、p38MAPK、CREB、ATF-2、转化生长因子β1(TGF-β1)、Smad2、Smad3、Smad4、CTGF的含量显著高于miR-342-5p和miR-423-5p低表达患者。结论:2型糖尿病患者体内高表达的miR-342-5p和miR-423-5p能够通过靶向调节p38MAPK、TGF-β1信号通路的方式来造成糖尿病肾病的发生。

Objective： To study the serum expression and clinical significance of miR-342-5p and miR-423-5p in type 2 diabetes mellitus patients with different urinary albumin excretion rate. Methods： A total of 126 patients with type 2 diabetes mellitus treated in our hospital between May 2013 and December 2015 were selected and divided into normal urine protein group（ NA group）,micro-urine protein group（ MI group） and macro-urine protein group（ MA group） according to the urinary albumin / creatinine ratio（ ACR）; 50 healthy volunteers were selected as control group. Serum was collected to determine the expression miR-342-5p and miR-423-5p as well as the content of p38 MAPK and TGF-β1 signaling pathway molecules. Results： Serum miR-342-5p and miR-423-5p expression of NA group,MI group and MA group were significantly higher than those of control group and the higher the ACR,the higher the serum miR-342-5p and miR-423-5p expression; serum MAPKKK,p38 MAPK,CREB,ATF-2,TGF-β1,Smad2,Smad3,Smad4 and CTGF content of patients with high miR-342-5p and miR-423-5p expression were significantly higher than those of patients with low miR-342-5p and miR-423-5p expression. Conclusions： Highly expressed miR-342-5p and miR-423-5p in patients with type2 diabetes mellitus can be targeted to adjust p38 MAPK and TGF-β1 signaling pathway to cause the occurrence of diabetic nephropathy.