糖尿病视网膜病变患者血清炎症因子和氧化应激指标的检测及意义

Detection and significance of serum inflammatory factors and oxidative stress in patients with diabetic retinopathy

目的:测定糖尿病视网膜病变(DR)患者血清炎症因子和氧化应激指标,探讨其在DR中的可能作用。方法:选取2015年6月~2016年6月于我院就诊的2型糖尿病患者116例作为研究对象,分为糖尿病无视网膜病变组(NDR组,n=63)和糖尿病伴视网膜病变组(DR组,n=53),选取同期我院体检中心健康体检者60例作为正常对照组(NC组),检测3组患者血管内皮生长因子(VEGF)、白介素(IL)-6、肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)以及超氧化物歧化酶(SOD)水平。结果:NC组、NDR组、DR组IL-6水平分别为(11.05±2.75)、(12.65±3.91)、(16.08±3.83)pg/m L,IL-6在NC组、NDR组、DR组中逐渐升高,差异有统计学意义(P<0.05)。NC组、NDR组、DR组TNF-α水平分别为(4.88±2.30)、(7.33±2.81)、(16.36±4.55)pg/m L,TNF-α在NC组、NDR组、DR组中逐渐升高,差异有统计学意义(P<0.05)。NC组、NDR组、DR组VEGF水平分别为(86.4±7.15)、(105.4±14.3)、(128.7±15.18)mmol/L,VEGF在NC组、NDR组、DR组中逐渐升高,差异有统计学意义(P<0.05)。NC组、NDR组、DR组MDA水平分别为(9.22±2.34)、(11.47±3.18)、(16.18±4.33)nmol/m L,MDA在NC组、NDR组、DR组中逐渐升高,差异有统计学意义(P<0.05)。NC组、NDR组、DR组SOD水平分别为(98.31±11.16)、(87.61±14.28)、(68.35±11.43)U/m L,SOD在NC组、NDR组、DR组中逐渐降低,差异有统计学意义(P<0.05)。结论:DR患者表达高水平的IL-6、TNF-α以及VEGF,同时DR患者体内存在明显的氧化应激,可见炎症反应及氧化应激状态对DR的发生发展具有重要作用。

Objective： To explore the possible role of serum inflammatory factors and oxidative stress in the pathogenesis of DR. Methods： A total of 116 patients with type 2 diabetes mellitus in our hospital from June 2015 to June 2016 were selected as the research objects,and they were divided into NDR group and DR group. At the same time,60 healthy controls were selected as NC group. The levels of IL-6,TNF-α,VEGF,MDA,and SOD were detected in the three groups. Results IL-6 levels of NC group,NDR group and DR group were（ 11. 05 ± 2. 75） pg / mL,（ 12. 65 ± 3. 91） pg / mL,（ 16. 08 ± 3. 83） pg / m L,IL-6 increased in NC group,NDR group and DR group,the difference is statistically significant（ P〈0. 05）. TNF-α levels of NC group,NDR group and DR group were（ 4. 88 ± 2. 30） pg / mL,（ 7. 33 ± 2. 81） pg / mL,（ 16. 36 ± 4. 55） pg / mL,TNF-α increased in NC group,NDR group and DR group,the difference is statistically significant（ P〈0. 05）. VEGF levels of NC group,NDR group and DR group were（ 86. 4 ± 7. 15）pg / mL,（ 105. 4 ± 14. 3） pg / mL,（ 128. 7 ± 15. 18） pg / mL,VEGF increased in NC group,NDR group and DR group,the difference is statistically significant（ P〈0. 05）. Conclusions： DR patients express high levels of IL-6,TNF-α and VEGF,while DR patients have obvious oxidative stress,so the inflammatory reaction and oxidative stress play an important role in the occurrence and development of DR.