单分子实时测序及其在微生物表观遗传学中的应用

Single molecule real time sequencing and its applications in microbial epigenetics—a review

表观遗传学对于微生物的生命进程起着重要作用。由限制-修饰系统调控的DNA修饰参与微生物的免疫防御系统,无限制-修饰系统调控的DNA修饰通过调控基因表达影响表型。然而,表观遗传信息还没有被常规地作为DNA信息收集分析。基于对DNA合成反应的动力学分析,单分子实时测序技术可以在获得基本序列数据的同时实现对被修饰核苷酸的检测。这个技术为微生物中已知DNA修饰的研究提供了新的平台,也为新型DNA修饰的发现做好准备。本文综述了单分子实时测序技术及其在微生物表观遗传学中的应用。

Epigenetics plays an important role in biological processes of microorganisms. DNA modification with restriction-modification system is a component of microbial immune system, and DNA modification without restriction system influences phenotypes by gene regulation. However, epigenetic information has not been collected for DNA sequence analysis routinely. Single molecule real time（SMRT） sequencing, based on analysis of DNA synthesis kinetics, enables the acquisition of DNA primary sequences and detection of modified nucleotides simultaneously. This technology provides a new platform for research on known DNA modifications and discovery of novel DNA modifications. This review summarized single molecule real time sequencing and its applications in microbial epigenetics.