普罗布考对动脉硬化闭塞症兔体内炎症反应的影响

Effect of probucol on inflammatory response in rabbits with arteriosclerosis obliterans

目的探讨普罗布考对动脉硬化闭塞症兔体内炎症反应的影响。方法采用右股动脉不全结扎在SPF级雄性健康新西兰白兔上建立动脉粥样硬化闭塞症动物模型。将模型兔分为实验组和对照组,其中实验组兔子采用普罗布考治疗。治疗4周后,采用HE染色评价各组兔股动脉血管的损伤程度;采用ELISA检测血浆中IL-1、IL-6、TNF-α及CRP水平;采用realtime PCR方法分析各组股动脉血管的IL-1、TNF-α的mRNA表达量;采用Western blot检测各组股动脉血管中NF-κB的核积累量及IκB磷酸化水平。结果实验组兔股动脉血管的损伤程度相比于对照组得到显著改善;实验组兔血清中IL-1、IL-6、TNF-α及CRP水平显著低于对照组;实验组兔股动脉血管中IL-1、TNF-α的mRNA表达水平、NF-κB的核积累量及IκB磷酸化水平显著低于对照组。结论普罗布考能够显著改善动脉硬化闭塞症兔体内的炎症反应。

Objective To explore the effect of probucol on the inflammatory response in rabbits with arteriosclerosis obliterans. Methods SPF male new zealand white rabbits were subjected to incomplete fight femoral artery ligation model of arteriosclerosis obliterans. The model rabbits were divided into experimental group and control group. The rabbits in experimental group were treated by probucol. After treatment for 4 weeks, HE staining was used to evaluate the injury severity of fight femoral artery in rabbits ; ELISA was used to measure the levels of IL-1 ,IL-6,TNF-α and CRP;Realtime PCR was used to detect the expressions of IL-1 ,TNF-α in right femoral artery;Western blot was used to measured the nucleus accumulations of NF-KB, and the phosphorylation of IKB. Results Compared with control group, the degree of pathological injury of right femoral artery was significantly atteunated, the levels of IL-1 ,IL-6 ,TNF-αand CRP in plasma, the mRNA ex- pressions of IL-1 ,TNF-α and the nucleus accumulations of NF-kB, and the phosphorylation of IkB in right femoral artery decreased signifi- cantly. Conclusion Probucol attenuates the inflammatory response in rabbits with arteriosclerosis obliterans significantly.