自由人槭‘秋焰’休眠芽的离体培养

In Vitro Culture of Dormant Bud of Acer×freemanii ‘Autumn Blaze'

为建立自由人槭‘秋焰’休眠芽的离体快繁体系,以其休眠芽(保留芽苞鳞片与剥去芽苞鳞片)为外植体,采用不同消毒方法和不同培养基进行离体启动和增殖培养研究。结果表明,带休眠芽茎段外植体用0.1%Hg Cl2消毒80~120s后,将芽苞鳞片剥去露出叶原基接种效果最佳,成活率可达80%以上。其休眠芽最适萌发培养基为改良MS+0.5mg/L 6-BA+0.02mg/L IBA+0.05mg/L ZT,萌发率可达64.44%;最适增殖培养基为改良MS+0.02mg/L IBA+0.01mg/L ZT,增殖系数达6.5。

To establish rapid-propagation system of in vitro culture of Acerxfreemanii‘Autumn Blaze＇, dormant buds of Acerxfreemanii‘ Autumn Blaze＇ was used as explants, and different sterilization method and different medium were used to conduct in vitro process and multipliction culture study. The results showed that the survival rate reached over 80% and the inoculation effect was the best when the stem explants were sterilized by 0. 1% HgCI2 with 80-120s, and then the bud scales was stripped to expose leaf primordium. The optimal induction medium was Improved MS＋0.5mg/L 6-BA＋0.02mg/L IBA＋0.05mg/L ZT, and the germination rate reached to 64.44%. The optimal proliferation medium was Improved MS＋0. 02 mg/L IBA＋0. 01 mg/L ZT, and the multiplication coefficient achieved to 6.5.