青蒿琥酯对人肝癌细胞HepG2增殖迁移及细胞凋亡的影响

Effects of artesunate on proliferation,migration and apoptosis of human hepatocarcinoma cell line,HepG2

目的利用多种生物学方法检测青蒿琥酯对肝癌细胞在增殖、周期、迁移、细胞凋亡等生物学特性方面的影响。方法在一定时间下,将不同浓度的青蒿琥酯(ART)作用于人肝癌细胞株HepG2,利用噻唑蓝(MTT)方法检测细胞增殖情况;流式细胞仪检测细胞周期和细胞凋亡变化情况;利用trans-well检测细胞迁移变化情况;利用聚合酶链反应(PCR)方法,检测ART用药前后与细胞凋亡相关的基因Caspase3的表达变化情况。结果对照组(0 mg/L ART)HepG2细胞在增殖、迁移等方面的能力显著高于青蒿琥酯实验组;对照组静息期(G0/G1期)细胞比例、细胞凋亡率显著低于ART实验组;ART实验组细胞Caspase3基因的表达水平显著高于对照组。结论实验结果表明ART是新的潜在的有效抗肝癌药物,可以通过抑制细胞增殖、迁移,阻滞细胞周期,提高细胞凋亡率等方面起到抑癌作用。

Objective To investigate the effects of artesunate on the proliferation, cycle, migration and apoptosis of hepatocarcinoma cells by multiple biological methods. Methods Artesunate （ART） was administered to human hepatocellular carcinoma cell line （HEPG2） at different concentrations for a certain time. MTT assay was used to determine the proliferation of cells. Flow cytometry was used to determine the changes of cell cycle and apoptosis. The change of cell migration was measured by transwell. The expression of apoptosis-related Caspase3 was determined by PCR before and after the use of ART. Results The proliferation and migration of HepG2 cells in the control group （0 mg/L ART） were significantly higher than those in the ART study group. In the control group, the percentage of cells at the resting phase （G0/G1 phase） and the cell apoptosis rate were significantly lower than those in the ART study group. The expression level of Caspase3 in the ART group was significantly higher than that in the control group. Conclusion The findings suggests that ART is a new potential and effective anti-hepatocarcinoma drug, which may inhibit cell proliferation and migration, arrest cell cycle and increase cell apoptosis rate.