摘要
目的 探究盐酸氨基葡萄糖对膝骨关节炎兔关节液中白细胞介素1（IL-1）、IL-6及肿瘤坏死因子α（TNF-α）水平的影响。 方法 选择成年雄性兔20只，随机分为 观察组和对照组，各10只。2组均制备右膝骨关节炎模型。对照组常规饮食，观察组于造模成功后在常规饮食的基础上喂食盐酸氨基葡萄糖80 mg/kg。给药喂养2个月后对比分析2组 双侧膝关节X线影像及右膝关节液中IL-1、IL-6、TNF-α水平。 结果 给药2个月后X线片显示观察组右膝关节肿胀不明显，而对照组关节肿胀明显。2组给药前右膝关节液中IL -1、IL-6、TNF-α水平比较差异无统计学意义（均P＞0.05）；给药2个月后，观察组IL-1、IL-6、TNF-α较给药前明显下降且明显低于同期对照组［（1.7±0.8）mg/L比（2.8± 0.8）、（3.7±0.8）mg/L，（0.82±0.27）mg/L比（1.32±0.43）、（1.98±0.46）mg/L，（2.06±0.24）mg/L比（3.08±1.17）、（4.13±1.25）mg/L］（均P＜0.05）。 结 论 盐酸氨基葡萄糖可降低膝骨关节炎模型兔关节液中IL-1、IL-6及TNF-α水平，进而缓解骨性病变与关节损伤。
Objective To explore the effect of glucosamine hydrochloride on interleukin 1（IL-1）, IL-6 and tumor necrosis factor α（TNF-α） in synovial fluid of knee osteoarthritis in rabbits. Methods Twenty adult male rabbits were set up as right knee osteoarthritis models and divided into observation group（n=10） and control group（n=10）. The control group was regularly fed; the observation group was added glucosamine hydrochloride 80 mg/kg for 2 months. Bilateral knee joints were scanned by X-ray; IL-6, IL-1 and TNF-α levels in synovial fluid of right knee joint were detected. Results Rats in observation group showed less visible swelling and inflammatory lesion of right knee joint in X-ray images compared to those in control group. Synovial fluid IL-6, IL-1 and TNF-α levels had no significant differences between groups before drug treatment（P〉0.05）; the levels in observation group after drug treatment were significantly lower than those before drug treatment and those in control group[（1.7±0.8）mg/L vs （2.8±0.8）,（3.7±0.8）mg/L; （0.82± 0.27）mg/L vs （1.32±0.43）,（1.98±0.46）mg/L; （2.06±0.24）mg/L vs （3.08±1.17）,（4.13±1.25）mg/L]（P〈0.05）. Conclusion Glucosamine hydrochloride can reduce IL-6, IL-1 and TNF-α levels in synovial fluid in knee osteoarthritis rabit, thus to alleviate bone lesion and joint injury.
出处
《中国医药》
2017年第1期126-129,共4页
China Medicine
基金
陕西省自然科学基金（2013C2047）
