二价金属离子对光棘球海胆免疫相关酶活力的影响

Effects of Divalent Metal Ions on Activities of Immune-related Enzymes in Sea Urchin Strongylocentrotus nudus

二价金属离子在海洋无脊椎动物免疫相关酶的活力调控中发挥重要作用。为了解二价金属离子对光棘球海胆免疫相关酶活力的影响,采用生化方法研究了8种二价金属离子在体外不同浓度下对光棘球海胆体腔液中酸/碱性磷酸酶、过氧化氢酶、超氧化物歧化酶、酚氧化酶和髓过氧化物酶活力的影响。结果显示,Cu^(2+)可明显增强碱性磷酸酶、过氧化氢酶、超氧化物歧化酶和酚氧化酶的活力;Mn^(2+)对酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶和髓过氧化物酶有强烈的激活作用;Zn^(2+)对超氧化物歧化酶和酚氧化酶有强烈的抑制作用;Fe^(2+)抑制过氧化氢酶和超氧化物歧化酶的活力;Ca^(2+)对酸性磷酸酶和髓过氧化物酶有抑制作用;Mg^(2+)对碱性磷酸酶有抑制作用;Cd^(2+)对超氧化物歧化酶和酚氧化酶有抑制作用;Pb^(2+)对过氧化氢酶、超氧化物歧化酶和酚氧化酶有抑制作用。试验结果表明,适宜浓度的Mn^(2+)和Cu^(2+)对光棘球海胆的非特异性免疫系统可能有促进作用,而Fe^(2+)、Zn^(2+)、Ca^(2+)、Mg^(2+)、Pb^(2+)和Cd^(2+)可能会削弱光棘球海胆的免疫应答能力。

In order to understand the effects of divalent metal ions on the activities of immune-related en- zymes in sea urchin Strongylocentrotus nudus, the eoelomic fluid of the sea urchin was incubated with Cu2＋ , Mn2＋ , Zn2＋ , Fe2＋ , Ca2＋ , Mg2＋ , Cd2＋ and Pb2＋ at different concentrations, respectively, and then the activities of acid phosphatase （ACP） and alkaline phosphatase （AKP）, catalase （CAT）, superoxide dismutase （SOD）, phenoloxidase （PO） and myeloperoxidase （MPO） in the incubation mixture were deter- mined with biochemical methods. The results showed that Cu2＋ led to increase the activities of AKP, CAT, SOD and PO; Mn2＋ enhanced the activities of ACP, AKP, CAT and MPO; Zn2＋ showed inhibitions on SOD and PO activities; Fe2＋ inhibited the activities of CAT and SOD; Ca2＋ inhibited the activities of ACP and MPO; Mg2＋ showed inhibition on AKP activities; Cd2＋ inhibited the activities of SOD and PO; Pb2＋ showed inhibitions on CAT, SOD and PO activities. The results implied that Mn2＋ and Cu2＋ promo- ted the immune-response capacity of the sea urchin at certain concentrations, while Fe2＋ , Zn2＋ , Ca2＋ , Mg2＋ , Pb2＋ and Cd2＋ might he harmful to the innate immune system in the sea urchin.