植物细胞壁组织化学定位染色方法和技术的比较研究

Comparative Study on Staining Methods and Techniques of Cell Wall Histochemistry

利用光学和荧光显微镜比较研究几种植物细胞壁组织化学定位染色方法和技术,结果表明:(1)硫酸消化法和硫酸氢黄连素—苯胺兰对染法研究凯氏带,对取材时间和部位要求高,建议两种方法配合使用,可相互印证是否具凯氏带;(2)苏丹7B染色法,蓝色激发光下不染色和硫酸氢黄连素—苯胺兰对染研究细胞壁栓质层3种方法中,不染色蓝色激发光下结果比苏丹7B染色法敏感显色,但苏丹7B染色法在普通光学显微镜下观察较为便捷;(3)木质化细胞壁染色方法中硫酸氢黄连素—苯胺兰对染法比间苯三酚—盐酸染色法易显色观察;(4)甲苯胺兰快速染色细胞壁取代常规苏丹Ⅲ/Ⅳ法,细胞边界和层次更清楚。

We carried out the comparative studies on several methods and techniques for cell wall histoehemistry under light and epifluorescence microscope. The results showed that： （1）To study Casparian bands using staining methods of sulphuric acid digestion and berberine hemisulfate-aniline, the requirements of material collecting time and part were high, therefore, it was recommended to use the two methods to mutual confirmation; （2）To study suberin lamellae using Sudan red 7B staining, and none staining and berberine hemisulfate-aniline blue staining under blue excited, none staining was more sensitive than Sudan red 7B, but Sudan red 7B staining is convenient under light microscope; （3）To study lignified walls, berberine hemisulfate- aniline staining was more sensitive than phloroglucinol-HC1; （4）Using TBO fast wall staining to replace Sudan m/IV conventional staining, the wall borders and layers were clearer.