党参米炒前后党参多糖与5-羟甲基糠醛的变化及其对胃肠平滑肌运动的影响

Content of polysaccharide and 5-hydroxymethyl furfural(5-HMF) in Codonopsis Radix after cooked with rice and its impact on rabbit gastrointestinal smooth muscle in vitro

目的探讨庙宇党参炮制(米炒)前后党参多糖及5-羟甲基糠醛(5-HMF)的量变化及其对家兔离体胃肠平滑肌运动的影响。方法运用苯酚硫酸法、高效液相色谱法分别对炮制前后党参多糖、5-HMF进行定量测定,采用BL-420F生物机能平滑肌实验系统,观察庙宇党参米炒前后对家兔离体胃肠平滑肌运动的影响;并考察党参米炒前后对工具药氯化乙酰胆碱(Ach)、新斯的明、氯化钡(BaCl_2)、阿托品、肾上腺素(Adr)所致胃肠平滑肌运动的干预作用。结果米炒党参多糖的量显著低于党参饮片(P<0.05),5-HMF的量显著高于党参饮片(P<0.01)。米炒党参显著兴奋离体胃肠平滑肌,缓解新斯的明、BaCl_2引起的强直性收缩,对抗Adr引起的肠管松弛(P<0.05)。结论党参米炒后5-HMF大量产生,能调控胃肠平滑肌的兴奋性收缩,再现5-HMF对离体胃肠平滑肌的效应,可能是米炒党参健脾功效增强的物质基础之一,其机制可能与其协同调控胃肠平滑肌N_2受体和β肾上腺素能受体有关。

Objective The effect of Codonopsis Radix was significantly different before and after being cooked, and to study the content ofpolysaccharide and 5-hydroxymethyl furfural （5-HMF） in Codonopsis Radix after cooked with rice and its impact on rabbit gastrointestinal smooth muscle in vitro. Methods Before and after being cooked, the content of polysaccharide and 5-HMF were determined with phenol sulfuric method and HPLC. BL-420F bio-functional experiment was applied to observe the impact of Codonopsis Radix processing with rice on rabbit gastrointestinal smooth muscle in vitro. Tests showed that acetylcholine （Ach）, neostigmine, barium chloride （BaCl2）, atropine, and adrenaline （Adr） intervened the relaxation of rabbit gastrointestinal smooth muscle in vitro. Results The content ofpolysaccharide was significantly lower than pieces after cooked with rice, and the content of 5-HMF was significantly higher. After processing it can further excite spontaneous activity of gastrointestinal smooth muscle in vitro. It can enhance bowel neostigmine and BaCl2 caused intestinal excitement and tension, objecting Adr caused intestinal relaxation. Conclusion 5-HMF was abundantly produced after Codonopsis Radix cooked with rice, caused excitability contraction on rabbit gastrointestinal smooth muscle in vitro, reproducing 5-HMF effect of gastrointestinal smooth muscle in vitro. It may be one of the material basis of Codonopsis Radix cooked with rice enhanced spleen. Its mechanism may be its collaborative control of N2R and βR results.