摘要
本研究采用RT-PCR方法,克隆长白猪(Landrace)GIP基因全长cDNA序列,并对其在家猪组织中的表达情况进行分析.结果显示:长白猪GIP(pGIP)cDNA全长435bp,编码144个氨基酸GIP的前体蛋白;该前体蛋白含有信号肽序列,经蛋白酶水解后产生GIP成熟肽.与人、小鼠GIP表达图谱类似,GIP mRNA也在长白猪小肠及肾脏中有较高水平表达.
The gastric inhibitory polypeptide/glucose-dependent insulinotropic polypeptide （GIP） shows the inhibitory effect on gastric acid secretion and incretin action. The aim of this study is to analyze the cDNA sequence of the pig GIP （pGIP） gene, and further examine the tissue expression of pGIP based on RT-PCR method. Result showed that pGIP cDNA is 532 bp in length and encodes a 144-amino acid GIP precursor which contains a signal peptide. RT-PCR assay showed that pGIP mRNA is highly expressed in kidney and gastrointestinal tract. Characterization of the pGIP gene will help to elucidate its physiological role.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第1期161-166,共6页
Journal of Sichuan University(Natural Science Edition)
基金
国家转基因生物新品种培育重大专项(2009zx08009-145B)
关键词
猪
GIP
分子克隆
结构分析
组织表迭
Pig
GIP
Molecular cloning
Structure analysis
Tissue expression.
