猪嗜淋巴细胞疱疹病毒2型GD27株gB基因生物信息学分析

Bioinformatics Analysis of gB Gene of Porcine Lymphotropic Herpesviruses Strain GD27

试验采用PCR方法扩增出猪嗜淋巴细胞疱疹病毒2型(porcine lymphotropic herpesviruses 2,PLHV-2)gB基因全序列,并进行测序,使用生物信息学软件对gB蛋白进行生物信息学分析和预测。结果显示,gB蛋白由876个氨基酸组成,分子式为C_(4500)H_(6975)N_(1199)O_(1351)S_(40),分子质量为100.77ku,等电点为6.45,不稳定系数为38.58;二级结构预测以无规则卷曲为主要结构,1-39位氨基酸为信号肽,761-780位氨基酸为跨膜区结构,具有多种功能位点,存在多处天然非规则结构蛋白;三级结构显示有一个具有溶解作用的环状构象。综合多种方法分析预测结果表明,gB基因存在多个B细胞线性抗原表位,可作为PLHV-2疫苗的候选抗原。

The glycoproteins B（gB）gene of porcine lymphotropic herpesviruses 2（PLHV-2）was amplified by PCR and sequenced,then bioinformatics analysis were conducted.The gB protein was composed of 876 amino acids,the molecular formula was C_（4500）H_（6975）N_（1199）O_（1351）S_（40）,the molecular mass was 100.77 ku,the value of theoretical isoelectric point was 6.45,and the instability index was 38.58.The prediction of secondary structure revealed that some alpha helixes and beta sheets exist in protein while random coil was major pattern.It had a signal peptide in the position1-39 amino acids and a transmembrane helice in the position 761-780 amino acids.It contained several prosites and several intrinsically disordered proteins.Tertiary structure model of gB protein showed a fusion loop.Multiple antigenic epitope located in gB through comprehensive analysis prediction method.It was suggested that gBgene could be as a candidate antigen for vaccination of PLHV-2.