上海地区屠宰场猪红斑丹毒丝菌的分离鉴定

Isolation and Identification of Erysipelothrix rhusiopathiae from Abattoirs in Shanghai Area

为了解上海地区猪红斑丹毒丝菌在健康猪群中的带菌状况,本试验选择上海地区标准化屠宰场的上市猪为研究对象,采集鼻拭子、肺脏、扁桃体和下颌淋巴结等样本,进行猪红斑丹毒丝菌的分离,采用VITEK 2Compact系统和VITEK MS快速鉴定系统进行鉴定,并设计1对16SrRNA基因引物,对分离菌株进行PCR扩增,随机选取16株分离菌进行测序及16SrRNA同源性分析。结果显示,49.07%(105/214)的样本中分离到猪红斑丹毒丝菌,分离菌株同参考菌株及疫苗株之间16SrRNA同源性为99.6%~100.0%。本试验结果表明上海地区猪群中确实存在猪红斑丹毒丝菌的健康带菌状况。

In order to comprehend the status of Erysipelothrix rhusiopathiae carried in swinery in Shanghai area,samples of pigs were collected from some standard abattoirs in Shanghai area,including nasal swab,lung,tonsil and mandibular lymph nodes.Strains were isolated from these samples for detecting the Erysipelothrix rhusiopathiae.Several kinds of methods were used,such as VITEK 2Compact system and VITEK MS system.A pair of primers was designed to amplify the 16 SrRNA gene.16 PCR products selected randomly were sequenced and 16 SrRNA homology analysis was conducted.As a result,strains were isolated from 49.07%（105/214）samples and were identified as Erysipelothrix rhusiopathiae.16 SrRNA homology in strains of isolation and reference were 99.6%to 100.0%.The result indicated that the situation was very severe for so many carriers of Erysipelothrix rhusiopathiae in health group in nursery.