玉米ZmCLCa基因克隆及其对氮素吸收的功能验证

Cloning of ZmCLCa Gene in Maize and Its Functional Characterization of Nitrogen Absorption

提高玉米的氮素吸收效率具有重要的意义。鉴于CLC蛋白家族具有运输NO-3的特性,本研究通过同源克隆的方法,克隆了玉米CLC家族基因Zm CLCa。该基因所编码的蛋白含有一个电压门控的氯离子通道(chloride channel)结构域,亚细胞定位结果显示该蛋白位于细胞膜上。在200 mmol/L的KNO3处理条件下,拟南芥转基因过表达株系中NO-3的含量明显高于野生型对照。因此,Zm CLCa基因很可能在玉米的氮素吸收利用过程中具有重要作用。

It has important significance to improve the efficiency of nitrogen absorption in maize. In consideration of the characteristics of transportation of NO3^- in CLC protein family, we used the means of homology-based cloning in this study to clone the CLC family genes ZmCLCa of maize. We found that the protein had a voltage-gated chlorine ion channel structure domain and subcellular localization result showed that the protein was located on the cell membrane. Under 200 mmol/L KNO3processing conditions,the content of NO3^- in transgenic Arabidopsis of strain was obviously higher than that in wild Arabidopsis. Therefore, ZmCLCa gene likely played an important role in the process of nitrogen absorption of maize.