MTDH与β-catenin在眼附属器淋巴瘤中的表达及意义

Expression and meaning of MTDH and β-catenin in ocular adnexal lymphoma

目的:探讨眼附属器淋巴瘤中MTDH与β-catenin的表达情况及临床意义。方法:收集青岛大学附属医院眼科1995-06/2015-12手术切除并确诊为眼附属器B细胞非霍奇金淋巴瘤(nonHodgkin lymphoma,NHL)的病理标本40例,以同期手术切除的眼部反应性淋巴组织增生组织20例作为对照组。采用PCR法检测淋巴瘤组与对照组中MTDH与β-catenin的mRNA表达情况;采用免疫组化法检测两组中MTDH与β-catenin的蛋白表达情况,并分析其与眼附属器淋巴瘤临床特征的关系。结果:与对照组相比,眼附属器淋巴瘤中MTDH与β-catenin的mRNA与蛋白表达均显著增加(P<0.05)。MTDH与β-catenin蛋白的阳性表达率与淋巴瘤的病理类型有关,随肿瘤恶性程度的增高,其表达增加(P<0.05),而与发病年龄、性别及部位无关(P>0.05)。在眼附属器淋巴瘤中,MTDH与β-catenin的蛋白表达呈正相关(r=0.389,P<0.05)。结论:MTDH与β-catenin的高表达可能与眼附属器淋巴瘤的发生发展有关,且两者表现为正相关。

AIM：To investigate the expression of MTDH and Bcatenin in ocular adnexal lymphoma lesions and its clinical significance.METHODS：Resected specimens were collected from patients suffering from B-cell non-Hodgkin lymphoma（NHL）（n= 40） and lymphadenosis（n = 20） of ocular adnexal in Affiliated Hospital of Qingdao University from June 1995 to December 2015.Lymphadenosis of ocular adnexal was acted as the control group.PCR and immunohistochemistry were employed to examine the MTDH and B-catenin mRNA and protein expression respectively.The relationship between the MTDH and Bcatenin protein expression level and the clinical pathological characteristics were analyzed.RESULTS：The expression of mRNA and protein of MTDH and B-catenin in ocular adnexal lymphoma lesions were higher than that in control group（P〈0.05）.The expression of MTDH and B-catenin proteins were related to pathologic type of tumors,but not related to age,gender or pathogenic site.With the increase of pathologic grade,MTDH and B-catenin labeling frequency increased gradually.And there was a positive correlation between MTDH and B-catenin（r=0.389,P=0.036）.CONCLUSION：Over expression of MTDH and B-catenin may play a significant role in the ocular adnexal lymphoma.The expression of MTDH and B-catenin has a positive relationship.