CyclinD1和Bax在甲状腺微小乳头状癌中的表达及意义

Expression and significance of CyclinD1 and Bax in papillary thyroid microcarcinoma

目的探究细胞周期蛋白D1(CyclinD1)和B淋巴细胞瘤-2相关X蛋白(Bax)蛋白在甲状腺微小乳头状癌中的表达及其价值。方法选取2014年1月至2015年6月该院病理科存档的甲状腺疾病手术病例石蜡标本,应用免疫组织化学(MaxvisionTM/HRP免疫组织化学染色法),以单克隆抗体兔抗人CyclinD1和鼠抗人Bax标记检测72例甲状腺微小乳头状癌、65例腺瘤组织和60例癌旁甲状腺正常组织,观察不同甲状腺组织中CyclinD1和Bax蛋白表达情况。结果 CyclinD1蛋白表达在甲状腺微小乳头状癌中的阳性率为79.17%,与腺瘤组织(χ~2=14.730,P<0.01)、癌旁甲状腺正常组织(χ~2=80.404,P<0.01)的表达差异有统计学意义,其与患者年龄、性别及淋巴结转移无明显关联(P>0.05)。Bax蛋白表达在甲状腺微小乳头状癌中的阳性率为66.67%,与腺瘤中的表达差异无统计学意义(P>0.05),与癌旁甲状腺正常组织的表达差异有统计学意义(P<0.01),其与淋巴结转移有关联(χ~2=4.598,P<0.05),与患者年龄、性别无明显关联(P>0.05)。甲状腺微小乳头状癌中CyclinD1与Bax蛋白表达呈正相关关联(χ~2=11.463,r=0.435,P<0.01)。结论 CyclinD1和Bax蛋白在甲状腺微小乳头状癌中高表达,与甲状腺微小乳头状癌的发生、发展相关,两者联合检测有助于对甲状腺微小乳头状癌的诊断。

Objective To explore the expression and value of CyclinD1 and Bax in papillary thyroid microcarcinoma.Methods The paraffin embedded specimens of thyroid diseases archived in the pathology department of our hospital from January 2014 to June 2015 were randomly selected.Expression of CyclinD1 and Bax were detected in 72 cases of papillary thyroid microcarcinoma,60 cases of paracancerous normal tissue and 65 cases of adenoma by using the monoclone antibody rabbit anti-CyclinD 1and rat anti-Bax labeling.Results The positive rate of CyclinD1 protein expression in papillary thyroid microcarcinoma was 79.17%,the difference in the expression between adenoma tissue and paracancerous normal tissue was statistical significant（χ~2=80.404,χ~2=14.730,P〈0.01）,which had no obvious correlation with the patient′s age,gender and lymph node metastasis（P〈0.05）.The positive rate of Bax protein expression in papillary thyroid microcarcinoma was 66.67%,its difference with adenoma was not obvious（P〈0.05）,but its difference with paracancerous normal tissue had statistical significance（P〈0.01）,which had correlation with lymph node metastasis（χ^2=4.598,P〈0.05）,while had no obvious correlation with the patient′s age and gender（P〈0.05）.The CyclinD1 expression in papillary thyroid microcarcinoma was positively correlated with the Bax protein expression（χ^2=11.463,r=0.435,P〈0.01）.Conclusion CyclinD1 and Bax protein are highly expressed in papillary thyroid microcarcinoma,which is related with the occurrence and development of papillary thyroid microcarcinoma.Their combined detection is conducive to diagnose papillary thyroid microcarcinoma.