鸡血藤原花青素的提取工艺和体外抗氧化活性

Extraction and antioxidant activity of Proanthocyanidins from Spatholobus suberectus Dunn

在单因素试验的基础上,运用响应面法优化鸡血藤Spatholobus suberectus Dunn原花青素的超声波辅助提取工艺。以料液比、丙酮体积分数、提取时间为考察因素,以原花青素的提取率为响应因子,进行Box-behnken中心组合试验,得到最佳提取工艺参数为:室温下,超声功率500 W,提取时间30 min,料液比1∶45(m/V),丙酮体积分数70%,提取1次。在此条件下,鸡血藤原花青素提取得率为25.59%。制得的原花青素对DPPH·、ABTS·的IC_(50)分别为8.42,10.55μg/m L。

Ultrasonic-assisted extraction （UAE ） was used to extract proanthocyanidins （PC ） from Spatholobus suberectus Dunn.Response surface methodology （RSM）,based on a three-factor,three-level Box-Behnken central composite design （BBD）,was employed to obtain the optimal extraction parame-ters,in which the extraction yields of PC was maximum.Three important factors,including extraction time,solid-liquid ratio and acetone concentration,were selected by single-factor tests.Optimized extrac-tion conditions for PC were a ratio of liquid to solid of 1∶45 mL/g,25 ℃,30 min,and acetone concen-tration as 70%,one-time extraction,500 W ultrasonic power.Under these conditions,the extraction yield of PC was 25.58%.In DPPH· and ABTS· scavenging assays,the IC50 of PC from S.suberectus were 8.42 μg/mL and 1 0.55 μg/mL,respectively.