婴儿喂养方式的改变对肠道菌群多样性的影响

Effects of feeding pattern changes on intestinal microflora diversity

目的分析婴儿喂养方式的改变对肠道菌群的影响。方法招募45名（月龄1-3个月）志愿者,随机分为三组（母乳组：母乳喂养;对照组：干预后市售普通配方奶粉喂养;产品组：干预后试验配方奶粉喂养）,利用平板计数和PCR-DGGE（变性梯度凝胶电泳）技术分析婴儿干预前和干预后其肠道菌群的变化。结果饮食干预后,婴儿肠道内双歧杆菌的数量,母乳组和产品组比较差异无统计学意义（t=0.510,P=0.614）,产品组和对照组比较差异存在统计学意义（t=2.541,P=0.016）;婴儿肠道内微生物的多样性指数,母乳组和产品组都显著增高（P≤0.05）。结论产品组配方奶粉是一款利于婴幼儿肠道有益菌增殖的产品。

Objective To study the effect of feeding pattern on infants＇ intestinal flora. Methods 45 volunteers （aged 1 to 3 months） were enrolled and randomly divided into three groups to accept breastfeeding （Breastfeeding group）, commercial infant formula （Control group） or the tested infant formula （Product group） respectively. Plate counting and PCR-DGGE （polymerase chain reaction-denaturing gradient gel electrophoresis） technology were used to analyze the intestinal flora of the infants before and after the diet intervention. Results No statistical difference （t=O. 510, P=0. 614） was found between the product group and breastfeeding group in bifidobacteria count after diet intervention, but between the product group and control group was statistical difference （t： 2. 541, P=0. 016） . The indexes of intestinal flora diversity in the product group and breastfeeding group significantly increased （P=0.05） . Conclusion The tested infant formula has positive effect on the growth of intestinal probiotic bacteria.