黄河鲤性别决定时间和相关基因表达研究

TIMING OF SEX DETERMINATION AND EXPRESSION ANALYSIS OF GENES IN YELLOW RIVER CARP(CYPRINUS CARPIO VAR.

鱼类性别决定和分化机制极为复杂,通过性腺组织切片鉴定得出黄河鲤从未分化性腺发育为Ⅱ期精巢、卵巢的时间为受精后第40天到第80天。选取一些可能参与黄河鲤性别决定分化相关的基因(amh、ar、cyp19a、cyp19b、dax1、dmrt1、er、foxl2、nobox、sox9a、sox9b、zp2)进行实时荧光定量PCR分析各个基因在受精后40d、45d、50d、55d、65d和80d的表达情况。结果显示性别决定相关基因在50d都有高表达,推测45—50 d为性别决定的关键时间。ar、amh、dax1、dmrt1、sox9a、sox9b六个基因在80d雄性表达量升高,且雄性明显高于雌性,推测这些基因参与精巢分化发育过程。cyp19a、cyp19b、foxl2、nobox、zp2五个基因在80d雌性表达升高,且高于雄性,推测其可能参与卵巢分化发育。

The mechanisms of fish sex determination and differentiation are complex. The current study investigated the morphology and the expression of genes related to sex determination differentiation during development of Yellow River carp. Tissue sections of gonad of Yellow River carp indicated that the time of gonad derived from undifferentiated to stage Ⅱ of testis or ovary were from 40 to 80 dpf（days post-fertilization）. The results showed that sex determination related genes（amh, ar, cyp19 a, cyp19 b, dax1, dmrt1, er, foxl2, nobox, sox9 a, sox9 b and zp2） highly expressed at 50 d, especially at 45—50 dpf. The expression ofar, amh, dax1, dmrt1, sox9 a, sox9 b increased at 80 dpf in males, which were significantly higher than those in females, suggesting that these genes might mediate male sexual differentiation. The expression of cyp19 a, cyp19 b, foxl2, nobox, zp2 increased at 80 d in females, which were higher than those in males, suggesting that they might regulate female sex differentiation.