抗心磷脂抗体、狼疮抗凝物、抗β_2-糖蛋白Ⅰ抗体对反复种植失败影响的研究

Study on the effect of ACA、LA and anti-β_2GPⅠantibody on the outcome of in repeated implantation failure

目的:探讨反复种植失败(RIF)与抗磷脂抗体(APA)包括抗心磷脂抗体(ACA)、狼疮抗凝物(LA)、抗β2-糖蛋白Ⅰ抗体(抗β2-GPⅠ抗体)的关系。方法:对96例RIF患者(研究组)和225例同期行体外受精-胚胎移植(IVF-ET)的患者(对照组),分别采用酶联免疫吸附法和部分凝血酶原时间法,联合检测静脉血清中的ACA、LA、抗β2-GPⅠ抗体。结果:研究组中ACA、LA、抗β2-GPⅠ抗体的阳性百分比,单个抗体检测和多个抗体联合检测均高于对照组,差异有统计学意义(P<0.05)。结论:ACA、LA、抗β2-GPⅠ抗体与RIF有关,对RIF患者,应常规筛查APA,以期对其早期干预,进而提高IVF-ET的临床妊娠率,降低流产率。

Objective To investigate the relationship between women with repeated implantation failure and anti-phospholipid antibodies,Including ACA、LA and anti-β2-GPⅠantibody. Method Levels of serum APA including ACA、LA and anti-β2-GPⅠantibody were determined by enzyme linked immune-absorbent assay and activated partial thromboplastin time method respectively in 96 patients with history of repeated implantation failure（study group） and 225 normal patients with IVF-ET（control group）. Results The positive rates of ACA、LA and anti-β2-GPⅠantibody in the study group were significantly higher than those in controls group（P〈0. 05）. Conclusion Levels of ACA、LA and anti-β2-GPⅠ antibody are associated with repeated implantation failure,Therefore routine screening of serum APA in patients with repeated implantation failure for the sake of early diagnosis and treatment,to improve the rate of pregnancy and reduce the abortion of IVF-ET.