谷红注射液对脑缺血大鼠大脑皮质线粒体功能的改善作用

Effect of Gu Hong Injection on the Function of Cerebral Cortex Mitochondria in Rats with Cerebral Ischemia

目的观察谷红注射液对缺血再灌注损伤大鼠大脑皮质线粒体功能的影响。方法采用线栓法制备SD大鼠大脑中动脉梗阻(MCAO)复灌模型,术后即刻给药,连续给药14天后通过神经功能缺损症状评分及贴纸去除实验评价药效。采用Western blot法检测线粒体自噬相关蛋白Beclin1、Parkin、KIFC2、SNAP-25及UCP3的表达变化。结果与模型组相比,谷红注射液能显著减轻缺血再灌注模型大鼠的神经功能缺损症状及恢复感觉运动功能,并有效逆转缺模型大鼠大脑皮质组织中线粒体与上述自噬相关蛋白表达的变化。结论谷红注射液改善脑缺血损伤的作用机制可能与激活线粒体自噬功能有关。

Objective To observe the effect of Gu Hong injection on the function of cerebral cortex mitochondria in rats with cere- bral iscbemia reperfusion injury. Methods The model of middle cerebral artery occlusion （MCAO） in SD rats was prepared by the meth- od of thread embolism. The drug was administered immediately after the operation, and the effect was evaluated by 14 days after continu- ous administration. The expression changes of mitochondrial autophagy related protein Beclinl, Parkin, KIFC2, SNAP - 25 and UCP3 were detected by Western blot. Results Compared with the model group, the Guhong injection can significantly reduce the symptoms of the neurological deficit in ischemia and reperfusion rat model and recovery of sensorimotor function, and effectively reverse the lack of changes in mitochondria and the autophagy related protein expression in cerebral cortex of the model rats. Conclusion It is possible that the action mechanism of Gu Hong injection on improving cerebral ischemic injury may be related to the activation of mitochondrial autophagy.