响应面法优化白蔹多糖的闪式提取工艺

Homogenate Extraction of Polysaccharides Ampelopsis Japonica( Thunb)Makino by Response Surface Methodology

目的:通过单因素实验筛选和响应面分析,确定闪式提取白蔹多糖的最优工艺条件。方法:以中药白蔹为原料,通过单因素试验,以料液比、电压、提取时间为自变量,确定主要影响因素;通过响应面分析法确定闪式提取白蔹多糖的最优工艺条件。结果白蔹多糖的闪式提取最优工艺条件为料液比(g/m L)1∶25,电压220 V,提取时间110 s,此条件下提取白蔹多糖的理论提取率为10.14%,验证实验中白蔹多糖的提取率为9.92%。结论:闪式提取法适合白蔹多糖的提取,该方法提取率高,提取时间少,具有快捷、方便的优点。

Objective：Through a single -factor experiment and the response surface analysis, the optimal technique process which is an extraction method of the homogenate extracting Ampelopsis japonica （Thunb） Makino polysaccharides was determined. Methods ： To determine the optimum process conditions of Ampelopsis japonica （Thunb） Makino polysac-charities homogenate extracting abased on response surface analysis, we chose Ampelopsis japonica （Thunb） Makino as raw material, the solid -liquid ratio, voltage and extraction time were considered as independent variable in single factor experiment. Results ： Optimal process conditions of Ampelopsis japonica （Thunb） Makino polysaeeharides with homogenate extraction are as follows： solid -liquid ratio （g/mL） was 1：25, the voltage was 220 V and the extraction time was 110 s, theoretical extrationrate Ampelopsisjaponica （ Thunb ） Makino polysaccharide was 1 0 . 1 4 % and the extraction rate was 9.92% in validation experiments. Conclusion：Homogenate extraction method was suitable for extracting Ampelopsis japonica （Thunb） Makino polysaccharides and this method has advantages in high extraction efficiency and less extraction time.