miR-19a和miR-19b在骨肉瘤中的表达及其临床意义

Expression of miR-19a and miR-19b and their clinical significance in osteosarcoma

目的:探讨miR-19a和miR-19b在骨肉瘤组织及配对瘤旁组织中的表达及其与临床病理特征的相关性。方法:收集32对骨肉瘤和配对的瘤旁组织,运用实时荧光定量PCR(qPCR)检测骨肉瘤组织及其瘤旁组织中miR-19a和miR-19b的表达,分析其与骨肉瘤临床病理特征的相关性及其临床意义。结果:qPCR结果显示骨肉瘤组织中miR-19a和miR-19b的平均表达量较瘤旁组织中均明显上调(P均<0.05)。二者的表达水平呈正相关(r=0.685,P=0.000)。miR-19a和miR-19b的表达与骨肉瘤病理分级之间存在正相关(r=0.478,P=0.027);miR-19a的表达与临床分期呈正相关(r=0.365,P=0.031)且与预后相关。Cox回归多因素分析发现miR-19a可作为骨肉瘤患者预后的影响因子(P=0.037)。结论:miR-19a和miR-19b在骨肉瘤中表达上调,在骨肉瘤的发生发展中可能发挥重要的作用,其中miR-19a可能作为骨肉瘤独立的预后标志物。

OBJECTIVE： To investigate the expression of miR-19a and miR-19b in osteosarcoma and adjacent non-cancerous tissues, and their correlation with clinicopathologic characteristics.METHODS： 32 cases of osteosarcoma tissues and paired adjacent non-cancerous tissues were collected, qPCR was used to detect the expressions of miR- 19a and miR-19b in the two tissues. Correlation between expression of the miRNA and clinical pathologic changes was performed. RESULTS： Expression of miR-19a and miR-19b was significantly up-regulated in osteosarcoma tissues as stage of osteosarcoma and related to the prognosis （r=0.685, P〈0.05）. Multivariate analyses suggested that miR-19a expression was an independent predictor for overall survival（P=0.037）. CONCLUSION： Our study showed that miR-19a and miR-19b may play an important role in the tumorigenesis and progression of osteosarcoma. In particular, miR-19a may serve as a prognostic marker and therapeutic target for osteosacoma.