小球藻片对大鼠的致畸试验

Teratogenic investigation of chlorella vulgaris tablet on Sprague Dawley rats

目的:评价小球藻片是否对SD大鼠具有致畸毒性,对其安全性进行毒理学评价。方法:孕鼠按体质量随机分为小球藻片低、中、高剂量组(分别为1 875、3 750、7 500mg/kg,相当于人服用量的12.5、25、50倍),阳性对照组(300mg/kg阿司匹林)和阴性对照组(等体积纯净水)。于孕期7-16 d灌胃给予受试物,试验期间称量孕鼠体质量,第20天处死。剖腹检查子宫连胚胎质量、着床数、活胎数、死胎数、吸收胎数、胎质量和体长、胎仔雌雄比例、胎仔外观、骨骼和内脏发育情况。结果:阳性对照组大鼠具有明显的母体毒性、胚胎毒性和致畸毒性。低、中、高剂量组小球藻片无母体毒性、胚胎毒性和致畸毒性。高剂量组出现少数胎仔外观畸形和骨骼畸形,畸形率与阴性对照组比较差异无统计学意义(P>0.05)。结论:在本实验条件下,小球藻片对SD大鼠无母体毒性、胚胎毒性和致畸毒性。

OBJECTIVE： The teratgenic and toxic effects of chlorella vulgaris tablets were evaluated in SD rats for health food safety concerns. METHODS： Pregnant rats were randomly assigned to chlorella vulgaris tablet groups （1 875,3 750,7 500 mg/kg）,positive control group （300 mg/kg aspirin） and negative control group. Rats were treated on the 7^th to the 16^th day during the gestation period and were killed on the 20^th day. Indicators for teratogenicity were examined：weight of uterus with embryo,fetal weight and body length,proportion of male and female fetuses,number of implantation,live fetuses,dead fetuses and reabsorbed fetuses,the morphology of fetuses,and abnormalities in the fetal skeletons and internal organs. RESULTS： Aspirin dose of 300 mg/kg produced maternal toxicity,embryonic toxicity and substantial teratogenic effects. The groups treated with doses of 1 875,3 750 and 7 500 mg/kg of the tablets did not produce maternal toxicity,embryonic toxicity nor teratogenicity. None of the observed effects was significantly different from the negative control group （P 〉 0.05）. CONCLUSION： Chlorella vulgaris tablet did not cause maternal toxicity,embryonic toxicity,or teratogenicity in pregnant rats.