胶孢炭疽菌CgSho1基因的克隆与功能分析

Gene cloning and functional analysis of CgSho1 in Colletotrichum gloeosporioides

HOG-MAPK(high osmolarity glycerol mitogen-activated protein kinase)信号途径是真菌MAPK途径中参与渗透压响应的一条重要通路,在植物病原菌生长发育及致病过程中发挥着重要的作用。Sho1(synthetic high osmolarity-sensitive protein1)是HOG-M APK信号途径上游的一个重要感受器,在不同真菌中常具有不同的功能。本研究从胶孢炭疽菌中克隆了Sho1的同源基因,命名为Cg Sho1,该基因编码一个291个氨基酸的蛋白,含有4个跨膜结构域和一个SH3功能域。利用同源重组的方法获得了该基因的敲除突变体,与野生型相比,敲除突变体表现为营养生长缓慢,菌丝稀疏且疏水性增强,产孢量下降,对氧化压力和渗透压更加敏感,致病力明显减弱。上述结果表明,Cg Sho1参与调控胶胞炭疽菌的营养生长、分生孢子产量、氧化应激反应、渗透压响应及致病性。

HOG-MAPK（high osmolarity glycerol mitogen-activated protein kinase） signal pathway involved in osmotic pressure response of MAPK pathway plays important role in growth development and pathogenicity of plant pathogens.Shol（synthetic high osmolarity-sensitive protein 1）,an important receptor in the upstream of HOG-MAPK signal pathway often has different functions in different fungi.In this study,the homologous gene of Shol in Colletotrichum gloeosporioides was cloned and named as CgSho1,which encodes a 291-amino acids protein,containing 4 transmembrane domains and a SH3 domain.The gene-knockout mutants of CgSho1 were obtained by homologous recombination.Comparing to wild type,the knockout mutants of CgSho1 showed slow growth,sparse aerial hyphae with increased hydrophobicity,decreased conidium production,more sensitive to oxidative stress and osmotic pressure,significantly decreased pathogenicity.These results demonstrated that Cg—Shol is involved in regulation of vegetative growth,conidium production,oxidative stress response,osmotic pressure response and pathogenicity of C.gloeosporioides.