皮肌炎中医证型与调节性T淋巴细胞的相关性

Relationship between TCM Syndrome and Regulatory T Cells in Peripheral Blood from Patients with Dermatomyositis

目的观察皮肌炎(DM)患者不同中医证型与外周血调节性T细胞(Treg)的关系。方法 149例确诊DM的患者分为热毒炽盛证、湿热蕴结证、寒湿痹阻证、脾肾两虚证、肝肾阴虚证,采用流式细胞术检测外周血Treg比例,研究其与中医证型病情活动相关性。并设118例健康对照组。结果热毒炽盛证、湿热蕴结证患者血Treg百分率显著低于其他组,差异有统计学意义(P<0.05);寒湿痹阻证血Treg百分率低于脾肾两虚证、肝肾阴虚证、健康对照组,差异有统计学意义(P<0.05);脾肾两虚证、肝肾阴虚证患者血Treg百分率与健康对照组的比较差异无统计学意义(P>0.05)。结论 DM患者的中医证型与其免疫功能状态存在关联。

Objective To demonstrate the relationship between different TCM Syndrome Types and regulatory T cells （Treg） in peripheral blood from patients with dermatomyositis. Methods One hundred and forty-nine cases of patients with dermatomyositis were enrolled and divided into heat-toxin flourishing syndrome, damp-heat accumulation syndrome, cold-dampness obstruction syndrome , Pi-Shen deficiency syndrome , and Gan-Shen yin deficiency syndrome. Flow cytometry was used to detect the percentage of Treg in peripheral blood from 149 patients and 118 healthy controls. Results The percentages of Treg cells in patients with heat-toxin flourishing syndrome, damp-heat accumulation syndrome was much lower than those in other groups （P 〈 0. 05） ; Also the percentages of Treg cells in patients with cold-dampness obstruction syndrome were significantly lower than those in Pi-Shen deficiency syndrome, Gan-Shen yin deficiency syndrome, and healthy controls, respectively（P 〈0.05 ）; There was no significant difference in percentages of Treg cells between Pi-Shen deficiency syndrome, Gan-Shen yin deficiency syndrome and healthy controls （P 〉 0. 05）. Conclusion There was an definite association between TCM Syndrome Types and regulatory T cells （Treg） in patients with dermatomyositis.