ARTP生物育种技术与装备研发及其产业化发展

Recent progress on atmospheric and room temperature plasma(ARTP) biobreeding technology, instrumentation and its industrialization

安全高效的微生物诱变和高通量筛选技术与装备是生物技术科研及产业发展的核心,具有重要的研究价值和广泛的应用前景。笔者研究团队成功将基于大气压射频辉光放电的常压室温等离子体(ARTP)应用于生物诱变育种领域,对其与生物大分子、细胞之间的作用机理进行了系统研究,并研发了具有自主知识产权的ARTP生物育种仪。因操作安全简便、突变快、突变率高、获得的突变体性状稳定等特点,ARTP育种仪已成功应用于百种以上微生物的诱变育种,在行业引起了广泛关注,并实现了的国际出口。综述了ARTP生物育种技术的原理、装备研发现状及其近年来的研究和应用进展,并对其今后的发展趋势进行了展望。

As the core of biotechnological research and industry, development of safe and efficient microbial mu-tagenesis technology and high-throughput screening as well as their instrumentation is of importance. Our research group has successfully developed a novel biological mutation breeding technology using atmospheric and room tem-perature plasma（ARTP） and invented the ARTP biobreeding equipment with our own intellectual property rights. Due to the benefits of easy operation, rapid mutation capability, high mutation rate, high mutant genetic stability and high safety for the operators, ARTP biobreeding equipment has attracted extensive attention in the industry and academy and has achieved international exports. It has been successfully applied in the breeding of more than 100 kinds of mi-croorganisms. This paper summarizes the recent progress on ARTP biobreeding technology and prospects.