台湾黑僵菌防治海榄雌瘤斑螟的菌株筛选

Selection of high efficient strains of Metarhizium anisopliae to control Acrobasis sp.

应用9株台湾黑僵菌菌株,筛选出对海榄雌瘤斑螟(Acrobasis sp.)具有高效防治的菌株,并开展林间应用研究。结果表明:在使用浓度为1×108孢子·m L-1的条件下,不同黑僵菌菌株对海榄雌瘤斑螟幼虫具有显著不同的致病力,其中黑僵菌F061菌株的致病力最强,在12 d的累积死亡率达98.75%,校正死亡率达97.56%±1.24%,均极显著的高于其他处理组(P<0.01),且对海榄雌瘤斑螟幼虫的致死中时最短,为7.25 d。在林间应用中,以1×109孢子·m L-1和2×109孢子·m L-1黑僵菌F061菌株处理对海榄雌瘤斑螟的防治效果最好,分别达到90.03%±1.74%和90.55%±1.21%,均极显著的高于其他处理(P<0.01)。从海榄雌瘤斑螟的防治效果结和应用成本考虑,选取1×109孢子·m L-1黑僵菌F061菌株可达到较好的防控效果。

From Taiwan,9 Metarhizium anisopliae strains were introduced to controll mangrove Acrobasis sp. and effects of forest application were compared. The results found that：（1） The pathogenicity of the 9 strains was different to controll Acrobasis sp.larvae,but F061 strains was the strongest as the death rate of Acrobasis sp. was 98.75% and the corrected death rate in laboratory12 d was 97.56% ±1.24%,which were significantly higher than other treatments（P〈 0.01）,and the LT50 was 7.25 d that was the shortest.（2） The best control effect of Acrobasis sp.,for the field application,was 1 × 109spore·m L-1and 2 × 109spore·m L-1of Metarhizium anisopliae F061 strains in mangrove,which were 90. 03% ± 1. 74% and 90. 55% ± 1. 21%,respectively,and were significantly higher than other treatment group（P 〈 0.01）. Finally,the 1×109spore·m L-1of F061 strains was suggested from the analysis of control effect and application cost.