针刺对一次离心运动后大鼠炎症反应的影响

Effect of Acupuncture on Rats Inflammation after a Single Eccentric Exercise

探究针刺干预对一次离心运动后大鼠炎症反应的影响。方法:114只雄性(SD)大鼠,随机分为4组:安静对照组(C)、单纯针刺组(A)、单纯运动组(E)、运动针刺组(EA),E组和EA组均在坡度-16°的跑台上进行一次离心运动,A组和EA组进行比目鱼肌部位斜刺,之后按0h、2h、24h、48h、72h、120h等时相进行取材。HE染色观察比目鱼肌细胞形态结构;Western Blot法分别检测比目鱼肌、股直肌中肿瘤坏死因子-α(TNF-α)的蛋白表达;Elisa法检测血浆中TNF-α的含量。结果 :1)HE染色发现C组和A组均无显著变化,E组出现不同程度的炎性细胞浸润,运动后进行针刺使炎性细胞浸润的峰值提前。2)比目鱼肌中TNF-α,A组与C组相比均无显著性差异(P>0.05);E组与C组、EA组具有显著性差异(P<0.05),在E组72h达到峰值,在EA组48h时相达到峰值。3)股直肌和血浆中TNF-α结果基本一致,A组与C组相比均无显著性差异(P>0.05);E组与C组具有显著性差异(P<0.05),E组与EA组无显著性差异(P>0.05)。结论:单纯进行针刺干预不会引起骨骼肌和血浆中明显的炎症反应,离心运动可以导致骨骼肌出现一定程度的炎症反应,离心运动后进行针刺干预使针刺部位骨骼肌炎症反应的程度增加,并在峰值之后促使其消退;针刺产生的作用是局部效果,对非针刺部位骨骼肌和血浆的炎症反应不会产生显著影响。

Objective：To explore the influence of acupuncture intervention on inflammatory response of rats after eccentric exercise.Methods：SD rats total 114 were randomly divided into four groups：control group（C）,eccentric exercise group（E）,acupuncture group（A）and sports acupuncture group（EA）.C group and EA group rats were submitted to eccentric exercise on a treadmill down at 16°decline.E group and EA group were conducted oblique thorn of triceps surae,then gaining the issue according to the 0h,2h,24 h,48h,72 h,120hphase.TNF-αprotein was examined in soleus and rectus femoris with Western Blot;the plasma TNF-αlevels were determined by Elisa.Results：（1）HE results,acupuncture group and control group showed no significant difference,exercise group with varying degrees of inflammatory cell infiltration,sports acupuncture making inflammatory cell infiltration of the peaks ahead.（2）In the soleus,A group showed no significant difference compared with the C group（P0.05）;E group showed significant difference compared with the C group and EA group（P0.05）,TNF-α,eccentric exercise group reaching its peak in 72 h,while exercise acupuncture group reaching its peak in 48h;（3）In the rectus femoris and soleus,A group showed no significant difference compared with the C group（P0.05）;E group showed significant difference compared with the C group（P0.05）and no significant difference compared with the EA group（P0.05）.Conclusions：Using the acupuncture could not have marked changes,eccentric exercise group caused muscle inflammation,and the exercise acupuncture could increase the degree of inflammation reaction and make it disappear after reaching the peak;acupuncture effect was local,and do not have a significant impact for the skeletal muscle of other parts and plasma.