摘要
为建立简单、稳定的丝瓜总酚提取和测定体系,对丝瓜果肉总酚的超声波辅助提取和福林-酚比色测定方法进行优化。丝瓜果肉与80%乙醇在比例为1∶10、40℃下超声波提取30min,总酚的提取效果较好。在测定波长750nm、0.5mol·L^(-1) Na2CO3体积3mL、0.5mol·L^(-1)福林-酚体积1.5mL、反应温度30℃、反应时间60min时,总酚含量与吸光度呈良好的线性关系:Y=0.6279 X+0.0273(R2=0.9947),说明此方法能够简便、准确、快速的测定丝瓜总酚含量。
To establish a simple and reliable extraction and measurement method for the total phenol content in luffa（Luffa cylindrical Roem）,ultrasound and Folin-Ciocaleu colorimetry were applied and operational procedures optimized.Phenols could be optimally extracted from the luffa mesocarp when it was mixed ultrasonically with 80%ethanol in 1∶10ratio at 40 ℃ for 30 min.The total phenolic content had a linear correlation（Y=0.6279 X＋0.0273;R2= 0.9947）with the optical absorbance at 750 nm when the solution was made with 3 mL of 0.5mol·L-1 Na2CO3 and 1.5mL of 0.5mol·L-1 Folin-Ciocalteu to react with the sample for 60 min at 30℃.The newly developed extraction and subsequent measurement procedures were simple,rapid,sensitive and accurate.It was considered adequate for the determination of total phenol in luffa.
出处
《福建农业学报》
CAS
北大核心
2016年第11期1204-1209,共6页
Fujian Journal of Agricultural Sciences
基金
福建省农业科学院科技创新团队PI项目(2016PI-40)
福建省自然科学基金项目(2015J01118)
国家大宗蔬菜产业体系福州试验站(CARS-25-G-20)
关键词
丝瓜
总酚
超声波
福林-酚
luffa
total phenol
ultrasound
folin-ciocalteu
