摘要
重力场流分离是最简单的场流分离( gravitational flow-field fractionation, GrFFF)技术,常用于分离粒径几微米到几十微米的颗粒及生物样品.利用自组装加工的重力场流分离仪器分离3种不同粒径(3、6、20μm)的聚 苯乙烯(PS)颗粒.自制了一种混合表面活性剂,并与商品化的表面活性剂FL-70进行了比较.通过均匀设计优化 流速、混合表面活性剂中聚乙二醇辛基苯基醚(Triton X-100)的质量分数、载液黏度、停流时间等分离条件,以分离 度(Rs)和保留比(R)为评价指标,发现FL-70的分离效能略优于自制的混合表面活性剂,可实现3种PS颗粒的完全分离(Rs1,为1. 771,Rs2为2. 074).结果表明该系统具有良好的分离性能.
Gravitational flow-field fractionation (GrFFF) is the simplest and cheapest field. flow fractionation technique. GrFFF utilizes earth's gravity as external field, providing a size. based separation. It can be applied to the separation and characterization of various micrometer. sized particles and biological samples. A GrFFF device was assembled, and three kinds of polystyrene (PS) particles with different sizes (20, 6 and 3 mu m) were used as the model particles. A homemade mixed surfactant was used and was compared with the surfactant FL-70. The separation conditions of GrFFF were optimized with uniform design, including flow rate, mass percentage of polyethylene glycol octylphenol ether (Triton X-100) in mixed surfactant, viscosity of carrier liquid and stop. flow time. The resolution (R-s) and retention ratio (R) were utilized as the evaluation indexes. The results suggest that the separation performance of FL-70 was slightly better than that of the mixed surfactant. Using FL-70 as the surfactant, particles of 20, 6 and 3 mu m were completely separated (with resolutions of 1.. 771 and 2.. 074). The results indicate that this system has good separation performance.
出处
《色谱》
CAS
CSCD
北大核心
2017年第2期216-221,共6页
Chinese Journal of Chromatography
关键词
重力场流分离
均匀设计
聚苯乙烯颗粒
载液
表面活性剂
流速
分离度
gravitational field-flow fractionation(GrFFF)
uniform design
polystyrene (PS) particles
carrier liquid
surfactant
flow rate
resolution